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OCT3/4 Monoclonal Antibody (EM92), eFluor™ 660, eBioscience™
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FIGURE: 1 / 7
OCT3/4 Antibody (50-5841-82) in Flow
Intracellular staining of F9 cells with 0.5 µg of Rat IgG2a K Isotype Control eFluor® 660 (Product # 50-4321-82) (blue histogram) or Anti-Human/Mouse OCT3/4 eFluor® 660 (purple histogram) using the Foxp3 Staining Buffer Set (Product # 00-5523-00) and protocol. Total cells were used for analysis.
Product Details
50-5841-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
EM92
Conjugate
Excitation/Emission Max
651/668 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The EM92 monoclonal antibody reacts with mouse and human Oct3/4, encoded by the Pou5F1 gene. Oct3/4 is a POU domain-containing transcription factor that is critical for maintaining embryonic stem (ES) and induced pluripotent stem (iPS) cells in a pluripotent state, and is expressed by ES, embryonic germ cells and embryonic carcinoma cell lines. In cells of the inner cell mass (ICM), reduction of Oct3/4 expression causes dedifferentiation to trophoectoderm, whereas increased expression results in differentiation to mesoderm and primitive endoderm. Oct3/4 regulates the expression of several genes, including FGF-4, UTF1, Sox2, Fbx15, Rex1 and osteopontin through distinct mechanisms. Furthermore, Oct3/4 frequently acts synergistically with Sox2 to regulate target gene expression, as is the case with FGF-4. It has been demonstrated that Oct3/4 expression in ES cells can be negatively regulated by either treatment with retinoic acid, or by removal of leukemia-inhibitory factor (LIF).
Applications Reported: This EM92 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This EM92 antibody has been tested by intracellular staining and flow cytometric analysis of the F9 cell line using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
POU5F1, also commonly known as Oct-4, is a maternally expressed octamer-binding protein that was the first transcription factor described for the early stages of development. The role of POU5F1 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing POU5F1, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: 3-Oct; 4-Oct; DADB-104B20.2; gp-9; HGNC:9221; MGC22487; NF-A3; Oct-3; Oct-4; OCT4-B1; OCT4B-variant 2; Octamer-binding protein 3; Octamer-binding protein 4; Octamer-binding transcription factor 3; OTF-3; OTTHUMP00000221150; OTTHUMP00000221151; POU class 5 homeobox 1; POU domain transcription factor OCT4; POU domain, class 5, transcription factor 1; pou-2; POU-type homeodomain-containing DNA-binding protein; pou2; transcriptional regulator
Gene Aliases: NF-A3; Oct-3; Oct-3/4; Oct-4; OCT3; Oct3/4; OCT4; OCT4Borf1; OTF-3; Otf-4; OTF3; Otf3-rs7; Otf3g; OTF4; POU5F1
UniProt ID: (Human) Q01860, (Mouse) P20263
Entrez Gene ID: (Human) 5460, (Mouse) 18999
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
RNA binding
protein binding
ubiquitin protein ligase binding
miRNA binding
sequence-specific DNA binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
sequence-specific double-stranded DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II transcription coactivator activity
chromatin binding
transcription corepressor activity
transcription factor binding
chromatin DNA binding
transcription regulatory region DNA binding
poly(A) RNA binding
protein heterodimerization activity
POU domain binding
HMG box domain binding
negative regulation of transcription from RNA polymerase II promoter
endodermal cell fate specification
blastocyst development
heart induction
endodermal-mesodermal cell signaling
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
response to wounding
anatomical structure morphogenesis
regulation of asymmetric cell division
regulation of gene expression
BMP signaling pathway
somatic stem cell population maintenance
positive regulation of transcription from RNA polymerase II promoter
cell fate commitment involved in formation of primary germ layer
cardiac cell fate determination
positive regulation of canonical Wnt signaling pathway
negative regulation of pri-miRNA transcription from RNA polymerase II promoter
mesodermal cell fate commitment
endodermal cell fate commitment
ectodermal cell fate commitment
trophectodermal cell differentiation
blastocyst growth
BMP signaling pathway involved in heart induction
transcription, DNA-templated
transcription from RNA polymerase II promoter
multicellular organism development
response to organic substance
stem cell population maintenance
germ-line stem cell population maintenance
response to retinoic acid
positive regulation of catenin import into nucleus
mRNA transcription from RNA polymerase II promoter
cell fate commitment
negative regulation of cell differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
negative regulation of calcium ion-dependent exocytosis
stem cell differentiation
positive regulation of protein kinase B signaling
negative regulation of protein kinase B signaling
positive regulation of SMAD protein import into nucleus
negative regulation of gene silencing by miRNA
regulation of heart induction by regulation of canonical Wnt signaling pathway
regulation of methylation-dependent chromatin silencing
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