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IL-4 Monoclonal Antibody (OX81), eFluor™ 660, eBioscience™
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FIGURE: 1 / 1
IL-4 Antibody (50-7045-80) in Flow
Rat splenocytes were cultured with Anti-Rat CD3 Functional Grade Purified (Product # 16-0030-85), Anti-Rat CD28 Functional Grade Purified (Product # 16-0280-81), Rat IL-2 Recombinant Protein (Product # 14-8020), and Rat IL-4 Recombinant Protein (Product # 14-8040) for 2 days followed by culture for an additional 3 days in Rat IL-2 and Rat IL-4 (right). The cells were then unstimulated (left) or stimulated (right) for 4 hours with the Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03) prior to fixation and ... View More
Product Details
50-7045-80
Applications
Tested Dilution
Publications
Product Specifications
Published species
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
OX81
Conjugate
Excitation/Emission Max
651/668 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
25 µg
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
Product Specific Information
Description: This OX81 monoclonal antibody reacts with rat interleukin (IL)-4. Also known as BCDF, BCGF-1 and BSF-1, IL-4 is secreted by activated Th2 and natural killer T cells. This cytokine is also produced by Th1 and mast cells albeit to a lesser extent. IL-4 is species-specific and promotes proliferation and differentiation of B cells. IL-4, in conjunction with other signals such as CD40, induces Ig isotype switching of B cells and the upregulation of MHC class II and CD23.
The OX81 antibody has been reported to exhibit neutralizing activity.
Applications Reported: This OX81 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This OX81 antibody has been tested by intracellular flow cytometric analysis of activated rat splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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