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Invitrogen

IL-10 Monoclonal Antibody (JES3-9D7), eFluor™ 660, eBioscience™

14 Published Figures
15 References
View all (101) IL-10 antibodies

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Product Details

50-7108-42

Applications
Tested Dilution
Publications

Immunohistochemistry (IHC)

-
View 2 publications 2 publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test
View 8 publications 8 publications

ELISA (ELISA)

-
View 4 publications 4 publications

Functional Assay (Functional)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), eFluor™ 660, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

JES3-9D7

Conjugate

eFluor™ 660 eFluor™ 660 eFluor™ 660

Excitation/Emission Max

651/668 nm View spectra spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11149363

Product Specific Information

Description: The JES3-9D7 monoclonal antibody reacts with human interleukin-10 (IL-10).

Applications Reported: This JES3-9D7 antibody has been reported for use in flow cytometric analysis, and intracellular staining followed by flow cytometric analysis.

Applications Tested: This JES3-9D7 antibody has been pre-titrated and tested by intracellular staining anf flow cytometryci analysis of Th2 polarized and stimulated human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Bioinformatics

Protein Aliases: CSIF; Cytokine synthesis inhibitory factor; H-IL-10; il 10; IL-10; IL10; ILN; Interleukin; Interleukin-10; Interleukin10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor

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Gene Aliases: CSIF; GVHDS; IL-10; IL10; IL10A; TGIF

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UniProt ID: (Human) P22301

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Entrez Gene ID: (Human) 3586

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Function(s)
cytokine activity interleukin-10 receptor binding protein binding growth factor activity protein dimerization activity
Process(es)
negative regulation of cytokine production positive regulation of cytokine production positive regulation of endothelial cell proliferation response to molecule of bacterial origin chronic inflammatory response to antigenic stimulus positive regulation of immunoglobulin production negative regulation of cytokine production involved in immune response negative regulation of chronic inflammatory response to antigenic stimulus positive regulation of B cell apoptotic process immune response negative regulation of cell proliferation response to xenobiotic stimulus regulation of gene expression negative regulation of autophagy response to activity response to inactivity hemopoiesis B cell differentiation leukocyte chemotaxis negative regulation of myeloid dendritic cell activation negative regulation of B cell proliferation response to lipopolysaccharide negative regulation of interferon-alpha production negative regulation of interferon-gamma production negative regulation of interleukin-1 production negative regulation of interleukin-12 production negative regulation of interleukin-18 production negative regulation of interleukin-6 production negative regulation of interleukin-8 production negative regulation of tumor necrosis factor production response to insulin negative regulation of heterotypic cell-cell adhesion positive regulation of heterotypic cell-cell adhesion response to carbon monoxide cellular response to hepatocyte growth factor stimulus type 2 immune response B cell proliferation negative regulation of T cell proliferation defense response to bacterium defense response to protozoan positive regulation of macrophage activation negative regulation of apoptotic process negative regulation of neuron apoptotic process negative regulation of nitric oxide biosynthetic process regulation of isotype switching negative regulation of MHC class II biosynthetic process positive regulation of MHC class II biosynthetic process positive regulation of cell cycle positive regulation of transcription, DNA-templated negative regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter positive regulation of JAK-STAT cascade negative regulation of inflammatory response regulation of synapse organization negative regulation of membrane protein ectodomain proteolysis response to glucocorticoid negative regulation of cytokine activity branching involved in labyrinthine layer morphogenesis cellular response to lipopolysaccharide cellular response to estradiol stimulus negative regulation of chemokine (C-C motif) ligand 5 production endothelial cell apoptotic process liver regeneration interleukin-10-mediated signaling pathway positive regulation of plasma cell differentiation positive regulation of pri-miRNA transcription from RNA polymerase II promoter regulation of response to wounding negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway regulation of complement-dependent cytotoxicity positive regulation of sprouting angiogenesis negative regulation of vascular smooth muscle cell proliferation positive regulation of vascular smooth muscle cell proliferation negative regulation of endothelial cell apoptotic process
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