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Invitrogen
Survivin Monoclonal Antibody (STLALYV), eFluor™ 660, eBioscience™
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FIGURE: 1 / 1
Survivin Antibody (50-9176-42) in Flow
TOP: Intracellular staining of unstimulated (left) or PHA-L-stimulated (right) normal human peripheral blood cells with Mouse IgG2b K Isotype Control eFluor® 660 (Product # 50-4732-82) (blue histogram) or Anti-Human Survivin eFluor® 660 (purple histogram) using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Total viable cells, as determined by Fixable Viability Dye eFluor® 450 (Product # 65-0863-14), were used for analysis. BOTTOM: Intracellular staining of PHA-L-stimulated, CFSE-labeled (Product # ... View More
Product Details
50-9176-42
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
STLALYV
Conjugate
Excitation/Emission Max
651/668 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This STLALYV monoclonal antibody recognizes human survivin (also known as Birc5). Survivin is a member of the inhibitor of apoptosis (IAP) family that also includes XIAP, c-IAP1, c-IAP2, and NAIP. Survivin functions to inhibit caspase activation, thereby leading to negative regulation of apoptosis. Survivin also plays a critical role in mitosis and cytokinesis where it is expressed only during G2/M and localizes to the mitotic spindle and is found at the cleavage furrow/midplate. Thus, survivin is highly expressed in tumors and in fetal tissue, but is absent in terminally-differentiated cells and non-proliferating cells. Deletion of survivin in developing thymocytes as well as in mature T cells showed that survivin is critical for cell division in lymphoid cells but not for inhibition of survival; the same is true for B lymphocytes.
Applications Reported: This STLALYV antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This STLALYV antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to BestProtocols®: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochrome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Survivin (IAP4) is the smallest member of the Inhibitors of Apoptosis Protein (IAP) gene family. The IAPs are involved in multiple cell functions such as cell signaling, cell division, metabolism, and exhibits differential expression in nearly all human cancers, but not in most normal tissues. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but the Survivin gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors yet low in adult tissues. Antisense transcripts are involved in the regulation of survivin's gene expression. Survivin is expressed in the G2/M phase of the cell cycle in a cycle-regulated manner. At the beginning of mitosis, survivin associates with microtubules of the mitotic spindle in a specific and saturable reaction that is regulated by microtubule dynamics. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptosis function and increased caspase-3 activity, a mechanism involved in cell death, during mitosis. Survivin may counteract a default induction of apoptosis in G2/M phase. Survivin is also abundantly expressed in brain tissues (astrocytes and some neurons) of adult rats following traumatic brain injury. Survivin has been found co-expressed with NeuN (mature neuronal marker) and PCNA (a cell cycle protein). Survivin might affect regulation of neural cell proliferative responses after brain injury. At least four transcript variants encoding distinct isoforms have been found for this gene, but the full-length natures of only three of them have been determined. The overexpression of survivin in cancer may overcome this apoptotic checkpoint and favor aberrant progression of transformed cells through mitosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: anti-apoptosis protein; IAP; Apoptosis inhibitor 4; Apoptosis inhibitor survivin; baculoviral IAP repeat-containing 5; Baculoviral IAP repeat-containing protein 5; BIRC 5; BIRC5; EPR 1; IAP; SVV; unnamed protein product
Gene Aliases: API4; BIRC5; EPR-1; IAP4
UniProt ID: (Human) O15392
Entrez Gene ID: (Human) 332
cysteine-type endopeptidase inhibitor activity
protein binding
microtubule binding
zinc ion binding
tubulin binding
enzyme binding
peptidase inhibitor activity
small GTPase binding
identical protein binding
protein homodimerization activity
cysteine-type endopeptidase inhibitor activity involved in apoptotic process
metal ion binding
protein heterodimerization activity
cobalt ion binding
chaperone binding
G2/M transition of mitotic cell cycle
mitotic cell cycle
mitotic cytokinesis
apoptotic process
mitotic spindle organization
chromosome segregation
mitotic spindle assembly checkpoint
sensory perception of sound
positive regulation of cell proliferation
protein complex localization
positive regulation of exit from mitosis
negative regulation of apoptotic process
negative regulation of transcription, DNA-templated
positive regulation of mitotic cell cycle
mitotic spindle midzone assembly
cell division
establishment of chromosome localization
positive regulation of mitotic cell cycle spindle assembly checkpoint
mitotic spindle assembly
positive regulation of mitotic sister chromatid separation
positive regulation of attachment of mitotic spindle microtubules to kinetochore
positive regulation of mitotic cytokinesis
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