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Invitrogen
CD25 (soluble) Monoclonal Antibody, PeproTech®
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FIGURE: 1 / 2
CD25 (soluble) Antibody (500-M02R-500UG) in WB
Western Blot: To detect Human sIL-2 Receptor alpha by Western Blot analysis CD25 (soluble) Monoclonal Antibody (Product # 500-M02R-500UG) can be used at a concentration of 0.20-0.40 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human sIL-2 Receptor alpha is 2.0-4.0 ng/lane, under non-reducing conditions.
Product Details
500-M02R-500UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
Class
Monoclonal
Type
Antibody
Immunogen
Hi-5 Insect cells-derived Recombinant Human sIL-2 Receptor alpha
Conjugate
Form
Lyophilized
Amount
500 µg
Purification
Protein G
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: ELCDDDPPEI PHATFKAMAY KEGTMLNCEC KRGFRRIKSG SLYMLCTGNS SHSSWDNQCQ CTSSATRNTT KQVTPQPEEQ KERKTTEMQS PMQPVDQASL PGHCREPPPW ENEATERIYH FVVGQMVYYQ CVQGYRALHR GPAESVCKMT HGKTRWTQPQ LICTGEMETS QFPGEEKPQA SPEGRPESET SCLVTTTDFQ IQTEMAATME TSIFTTEYQ.
Preparation: Produced in mice immunized with highly pure Recombinant Human sIL-2 Receptor alpha. Anti-Human sIL-2 Receptor alpha-specific antibody was purified from cell culture by Protein G affinity chromatography.
Target Information
CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD25; IL 2 RA; IL 2 receptor; IL 2R; IL 2R subunit alpha; il-2 receptor alpha; IL-2 receptor subunit alpha; IL-2R subunit alpha; IL2 RA; IL2 RA antibody; IL2 receptor; IL2 receptor subunit alpha; IL2R subunit alpha; Il2ra antibody; interleukin 2 receptor, alpha; interleukin-2 receptor alpha; interleukin-2 receptor precursor; Interleukin-2 receptor subunit alpha; Interleukin2 receptor subunit alpha; p55; sIL 2R; soluble IL 2 receptor; TAC antigen; unnamed protein product
Gene Aliases: CD25; IDDM10; IL2R; IL2RA; IMD41; p55; TCGFR
UniProt ID: (Human) P01589
Entrez Gene ID: (Human) 3559
immune system process
inflammatory response to antigenic stimulus
regulation of T cell tolerance induction
regulation of immune system process
apoptotic process
activation-induced cell death of T cells
inflammatory response
immune response
cell surface receptor signaling pathway
Notch signaling pathway
interleukin-2-mediated signaling pathway
positive regulation of T cell proliferation
positive regulation of activated T cell proliferation
negative regulation of T cell proliferation
T cell homeostasis
positive regulation of T cell differentiation
regulation of T cell homeostatic proliferation
lymphocyte proliferation
negative regulation of lymphocyte proliferation
negative regulation of inflammatory response
activated T cell proliferation
regulation of CD4-positive, alpha-beta T cell proliferation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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