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Invitrogen
IL-10 Monoclonal Antibody, PeproTech®
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FIGURE: 1 / 3
IL-10 Antibody (500-M128-500UG) in WB
Western Blot: To detect Murine IL-10 by Western Blot analysis IL-10 Monoclonal Antibody (Product # 500-M128-500UG) can be used at a concentration of 1.0-2.0 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for rRecombinant Murine IL-10 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.
Product Details
500-M128-500UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
Class
Monoclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Murine IL-10
Conjugate
Form
Lyophilized
Amount
500 µg
Purification
Protein G
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: MSRGQYSRED NNCTHFPVGQ SHMLLELRTA FSQVKTFFQT KDQLDNILLT DSLMQDFKGY LGCQALSEMI QFYLVEVMPQ AEKHGPEIKE HLNSLGQKLK TLRMRLRRCH RFLPCENKSK AVEQVKSDFN KLEDQGVYKA MNEFDIFINC IEAYMMIKMK S.
Preparation: Produced in Sprague Dawley rats immunized with highly pure Recombinant Murine IL-10. Anti-Murine IL-10-specific antibody was purified from cell culture by Protein G affinity chromatography.
Target Information
IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CSIF; Cytokine synthesis inhibitory factor; H-IL-10; il 10; IL-10; ILN; Interleukin; Interleukin-10; Interleukin10; MGC126450; MGC126451; RP11-262N9.1
Gene Aliases: CSIF; Il-10; Il10
UniProt ID: (Mouse) P18893
Entrez Gene ID: (Mouse) 16153
negative regulation of cytokine production
response to molecule of bacterial origin
negative regulation of cytokine secretion involved in immune response
negative regulation of chronic inflammatory response to antigenic stimulus
positive regulation of B cell apoptotic process
inflammatory response
immune response
regulation of gene expression
negative regulation of myeloid dendritic cell activation
negative regulation of B cell proliferation
negative regulation of interferon-gamma production
negative regulation of interleukin-12 production
negative regulation of interleukin-6 production
negative regulation of tumor necrosis factor production
receptor biosynthetic process
negative regulation of tumor necrosis factor biosynthetic process
defense response to bacterium
defense response to protozoan
negative regulation of apoptotic process
negative regulation of growth of symbiont in host
negative regulation of nitric oxide biosynthetic process
positive regulation of MHC class II biosynthetic process
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of JAK-STAT cascade
positive regulation of cytokine secretion
negative regulation of inflammatory response
negative regulation of membrane protein ectodomain proteolysis
positive regulation of sequence-specific DNA binding transcription factor activity
response to glucocorticoid
regulation of sensory perception of pain
negative regulation of cytokine activity
branching involved in labyrinthine layer morphogenesis
cellular response to lipopolysaccharide
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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