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Invitrogen

CCL5 (RANTES) Monoclonal Antibody, PeproTech®

5 Published Figures
14 References
View all (36) CCL5 (RANTES) antibodies

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CCL5 (RANTES) Monoclonal Antibody, PeproTech®

Product Details

500-M75-500UG

Applications
Tested Dilution
Publications

Western Blot (WB)

0.5-1.0 µg/mL
View 1 publication 1 publication

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

ELISA (ELISA)

2.0-4.0 µg/mL
View 5 publications 5 publications

Neutralization (Neu)

-
View 1 publication 1 publication

In vitro Assay (In vitro)

-
View 2 publications 2 publications

Immunostaining (IS)

-
View 4 publications 4 publications
Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse

Class

Monoclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human RANTES (CCL5)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Amount

500 µg

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2929127

Product Specific Information

AA Sequence of recombinant protein: SPYSSDTTPC CFAYIARPLP RAHIKEYFYT SGKCSNPAVV FVTRKNRQVC ANPEKKWVRE YINSLEMS.

Preparation: Produced in BALB/c mice immunized with highly pure Recombinant Human RANTES (CCL5). Anti-Human RANTES (CCL5)-specific antibody was purified from ascites fluid by Protein A affinity chromatography.

Sandwich ELISA: Assuming 100 µL/well, a concentration of 2.0-4.0 µg/mL of this antibody will detect at least 500 pg/mL of Recombinant Human RANTES (CCL5) when used with PeproTech Biotinylated Antigen Affinity Purified Anti-Human RANTES (CCL5) (500-P36BT) as the detection antibody at a concentration of approximately 0.5-1.0 µg/mL.

Western Blot: To detect Human RANTES (CCL5) by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human RANTES (CCL5) is 0.25-2.0 ng/lane, under reducing conditions and 0.25-0.5 ng/lane, under non-reducing conditions.

Target Information

CCL5 (RANTES) is a chemokine gene located on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. CCL5, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells, and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms. Diseases associated with CCL5 include Human Immunodeficiency Virus Type 1 and Periapical Granuloma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: beta-chemokine RANTES; C-C motif chemokine 5; chemokine (C-C motif) ligand 5; EoCP; Eosinophil chemotactic cytokine; MGC17164; OTTHUMP00000197106; regulated upon activation, normally T-expressed, and presumably secreted; SIS δ; SIS-delta; SISδ; small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T cell-specific protein precursor; T-cell specific protein p288; T-cell-specific protein RANTES; TCP228; unnamed protein product

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Gene Aliases: CCL5; D17S136E; eoCP; RANTES; SCYA5; SIS-delta; SISd; TCP228

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UniProt ID: (Human) P13501

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Entrez Gene ID: (Human) 6352

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Function(s)
phosphatidylinositol phospholipase C activity protein kinase activity cytokine activity protein binding chemokine activity phospholipase activator activity receptor signaling protein tyrosine kinase activator activity CCR1 chemokine receptor binding CCR4 chemokine receptor binding CCR5 chemokine receptor binding chemoattractant activity chemokine receptor binding identical protein binding protein homodimerization activity chemokine receptor antagonist activity CCR chemokine receptor binding
Process(es)
dendritic cell chemotaxis monocyte chemotaxis regulation of chronic inflammatory response calcium ion transport cellular calcium ion homeostasis exocytosis chemotaxis inflammatory response immune response leukocyte cell-cell adhesion G-protein coupled receptor signaling pathway cell-cell signaling response to virus response to toxic substance positive regulation of macrophage chemotaxis positive regulation of T cell chemotaxis positive regulation of smooth muscle cell migration positive regulation of cell migration phospholipase D-activating G-protein coupled receptor signaling pathway positive regulation of TOR signaling positive regulation of cell-cell adhesion mediated by integrin response to cytokine positive regulation of homotypic cell-cell adhesion response to tumor necrosis factor chemokine (C-C motif) ligand 5 signaling pathway positive regulation of T cell proliferation neutrophil activation negative regulation by host of viral transcription cellular response to fibroblast growth factor stimulus positive regulation of viral genome replication negative regulation of viral genome replication positive regulation of innate immune response negative regulation of G-protein coupled receptor protein signaling pathway positive regulation of G-protein coupled receptor protein signaling pathway positive regulation of cell adhesion positive regulation of translational initiation eosinophil chemotaxis macrophage chemotaxis positive regulation of smooth muscle cell proliferation epithelial cell proliferation positive regulation of epithelial cell proliferation regulation of insulin secretion regulation of T cell activation positive chemotaxis positive regulation of protein kinase B signaling positive regulation of calcium ion transport antimicrobial humoral immune response mediated by antimicrobial peptide chemokine-mediated signaling pathway negative regulation of chemokine-mediated signaling pathway negative regulation of T cell apoptotic process positive regulation of T cell apoptotic process ERK1 and ERK2 cascade cellular response to interferon-gamma cellular response to interleukin-1 cellular response to tumor necrosis factor positive regulation of monocyte chemotaxis receptor signaling pathway via STAT cellular response to virus positive regulation of neuroinflammatory response positive regulation of STAT cascade negative regulation of macrophage apoptotic process positive regulation of T cell migration positive regulation of natural killer cell chemotaxis
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