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Invitrogen
CCL5 (RANTES) Monoclonal Antibody, PeproTech®
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CCL5 (RANTES) Antibody (500-M75-500UG) in WB
Western Blot: To detect Human RANTES (CCL5) by Western Blot analysis CCL5 (RANTES) Monoclonal Antibody (Product # 500-M75-500UG) can be used at a concentration of 0.5-1.0 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human RANTES (CCL5) is 0.25-2.0 ng/lane, under reducing conditions and 0.25-0.5 ng/lane, under non-reducing conditions.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
500-M75-500UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
Class
Monoclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human RANTES (CCL5)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
1 mg/mL
Amount
500 µg
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: SPYSSDTTPC CFAYIARPLP RAHIKEYFYT SGKCSNPAVV FVTRKNRQVC ANPEKKWVRE YINSLEMS.
Preparation: Produced in BALB/c mice immunized with highly pure Recombinant Human RANTES (CCL5). Anti-Human RANTES (CCL5)-specific antibody was purified from ascites fluid by Protein A affinity chromatography.
Sandwich ELISA: Assuming 100 µL/well, a concentration of 2.0-4.0 µg/mL of this antibody will detect at least 500 pg/mL of Recombinant Human RANTES (CCL5) when used with PeproTech Biotinylated Antigen Affinity Purified Anti-Human RANTES (CCL5) (500-P36BT) as the detection antibody at a concentration of approximately 0.5-1.0 µg/mL.
Western Blot: To detect Human RANTES (CCL5) by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human RANTES (CCL5) is 0.25-2.0 ng/lane, under reducing conditions and 0.25-0.5 ng/lane, under non-reducing conditions.
Target Information
CCL5 (RANTES) is a chemokine gene located on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. CCL5, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells, and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms. Diseases associated with CCL5 include Human Immunodeficiency Virus Type 1 and Periapical Granuloma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: beta-chemokine RANTES; chemokine (C-C motif) ligand 5; eosinophil chemotactic cytokine; MGC17164; OTTHUMP00000197106; regulated upon activation, normally T-expressed, and presumably secreted; SIS δ; SISδ; small inducible cytokine subfamily A (Cys-Cys), member 5; T cell-specific protein precursor; T-cell specific protein p288; unnamed protein product
Gene Aliases: D17S136E; eoCP; RANTES; SCYA5; SIS-delta; SISd; TCP228
UniProt ID: (Human) P13501
Entrez Gene ID: (Human) 6352
phosphatidylinositol phospholipase C activity
protein kinase activity
protein binding
chemokine activity
phospholipase activator activity
receptor signaling protein tyrosine kinase activator activity
CCR1 chemokine receptor binding
CCR4 chemokine receptor binding
CCR5 chemokine receptor binding
chemoattractant activity
chemokine receptor binding
identical protein binding
protein homodimerization activity
chemokine receptor antagonist activity
CCR chemokine receptor binding
dendritic cell chemotaxis
monocyte chemotaxis
regulation of chronic inflammatory response
calcium ion transport
cellular calcium ion homeostasis
exocytosis
chemotaxis
inflammatory response
immune response
leukocyte cell-cell adhesion
G-protein coupled receptor signaling pathway
cell-cell signaling
response to virus
response to toxic substance
positive regulation of macrophage chemotaxis
positive regulation of T cell chemotaxis
positive regulation of smooth muscle cell migration
positive regulation of cell migration
phospholipase D-activating G-protein coupled receptor signaling pathway
positive regulation of TOR signaling
positive regulation of cell-cell adhesion mediated by integrin
positive regulation of homotypic cell-cell adhesion
chemokine (C-C motif) ligand 5 signaling pathway
positive regulation of T cell proliferation
neutrophil activation
negative regulation by host of viral transcription
cellular response to fibroblast growth factor stimulus
positive regulation of viral genome replication
negative regulation of viral genome replication
positive regulation of innate immune response
negative regulation of G-protein coupled receptor protein signaling pathway
positive regulation of G-protein coupled receptor protein signaling pathway
positive regulation of cell adhesion
positive regulation of translational initiation
eosinophil chemotaxis
macrophage chemotaxis
positive regulation of smooth muscle cell proliferation
regulation of insulin secretion
regulation of T cell activation
positive chemotaxis
positive regulation of protein kinase B signaling
positive regulation of calcium ion transport
antimicrobial humoral immune response mediated by antimicrobial peptide
chemokine-mediated signaling pathway
negative regulation of chemokine-mediated signaling pathway
negative regulation of T cell apoptotic process
positive regulation of T cell apoptotic process
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to tumor necrosis factor
positive regulation of monocyte chemotaxis
receptor signaling pathway via STAT
cellular response to virus
positive regulation of neuroinflammatory response
positive regulation of STAT cascade
negative regulation of macrophage apoptotic process
positive regulation of T cell migration
positive regulation of natural killer cell chemotaxis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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