Invitrogen

IFN gamma Monoclonal Antibody, PeproTech®

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IFN gamma Monoclonal Antibody, PeproTech®

Product Details

500-M90-500UG

Applications
Tested Dilution
Publications

Western Blot (WB)

0.20-0.40 µg/mL
-

ELISA (ELISA)

4.0-8.0 µg/mL
-

Neutralization (Neu)

-
View 1 publication 1 publication

In vitro Assay (In vitro)

-
View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-
View 3 publications 3 publications
Product Specifications

Species Reactivity

Human

Published species

Bacteria, Human, Mouse

Host/Isotype

Mouse

Class

Monoclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human IFN-gamma
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_3074172

Product Specific Information

AA Sequence of recombinant protein: MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFQGR RASQ.

Preparation: Produced in BALB/c mice immunized with highly pure Recombinant Human IFN-gamma. Anti-Human IFN-gamma-specific antibody was purified from cell culture by Protein A affinity chromatography.

Sandwich ELISA: Assuming 100 µL/well, a concentration of 4.0-8.0 µg/mL of this antibody will detect at least 500 pg/mL of Recombinant Human IFN-gamma when used with PeproTech Biotinylated Antigen Affinity Purified Anti-Human IFN-gamma (500-P32BT) as the detection antibody at a concentration of approximately 2.0- 4.0 µg/mL.

Western Blot: To detect Human IFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IFN-gamma is 1.0-2.0 ng/lane, under reducing or non-reducing conditions.

Target Information

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: H-IFN-g; IFN γ; IFN-gamma; IFN-y; IFNγ; Immune interferon; Interferon; Interferon gamma; Interferon y; Interferon γ; interferon, gamma; Interferonγ; M-IFN-g; R-IFN-g

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Gene Aliases: IFG; IFI; IFNG

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UniProt ID: (Human) P01579

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Entrez Gene ID: (Human) 3458

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Function(s)
cytokine activity interferon-gamma receptor binding cytokine interferon superfamily intercellular signal molecule
Process(es)
protein import into nucleus, translocation negative regulation of transcription from RNA polymerase II promoter neutrophil apoptotic process regulation of the force of heart contraction adaptive immune response CD8-positive, alpha-beta T cell differentiation involved in immune response apoptotic process movement of cell or subcellular component humoral immune response cell cycle arrest cell surface receptor signaling pathway positive regulation of cell proliferation response to virus positive regulation of autophagy positive regulation of gene expression negative regulation of gene expression positive regulation of epithelial cell migration antigen processing and presentation neutrophil chemotaxis negative regulation of epithelial cell differentiation endoplasmic reticulum unfolded protein response negative regulation of myelination positive regulation of synaptic transmission, cholinergic negative regulation of interleukin-17 production positive regulation of interleukin-12 production positive regulation of interleukin-23 production positive regulation of tumor necrosis factor production positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation involved in immune response positive regulation of peptidyl-serine phosphorylation of STAT protein positive regulation of smooth muscle cell apoptotic process positive regulation of T cell proliferation response to drug positive regulation of tyrosine phosphorylation of Stat1 protein defense response to bacterium defense response to protozoan negative regulation of growth of symbiont in host positive regulation of chemokine biosynthetic process positive regulation of interleukin-12 biosynthetic process positive regulation of MHC class II biosynthetic process positive regulation of interleukin-6 biosynthetic process positive regulation of nitric oxide biosynthetic process positive regulation of neuron differentiation positive regulation of osteoclast differentiation positive regulation of transcription from RNA polymerase II promoter positive regulation of isotype switching to IgG isotypes negative regulation of smooth muscle cell proliferation positive regulation of interleukin-1 beta secretion regulation of insulin secretion T cell receptor signaling pathway sensory perception of mechanical stimulus positive regulation of membrane protein ectodomain proteolysis defense response to virus positive regulation of killing of cells of other organism interferon-gamma-mediated signaling pathway regulation of interferon-gamma-mediated signaling pathway positive regulation of fructose 1,6-bisphosphate 1-phosphatase activity positive regulation of fructose 1,6-bisphosphate metabolic process positive regulation of vitamin D biosynthetic process positive regulation of calcidiol 1-monooxygenase activity cellular response to lipopolysaccharide cellular response to interleukin-18 positive regulation of establishment of protein localization to plasma membrane extrinsic apoptotic signaling pathway regulation of neuronal action potential positive regulation of exosomal secretion positive regulation of tumor necrosis factor (ligand) superfamily member 11 production regulation of hepatocyte proliferation
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