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          • Primary Antibodies ›
          • VEGF-165 Antibodies

          Invitrogen

          VEGF-165 Polyclonal Antibody, Biotin, PeproTech®

          View all (12) VEGF-165 antibodies

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          Cite VEGF-165 Polyclonal Antibody, Biotin, PeproTech®

          Additional Information:
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          • Antibody Testing Data (3)
          VEGF-165 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          VEGF-165 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          VEGF-165 Antibody (500-P10GBT-25UG) in WB

          Western Blot: To detect hVEGF by Western Blot analysis VEGF-165 Polyclonal Antibody, Biotin (Product # 500-P10GBT-500UG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hVEGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          VEGF-165 Antibody in Western Blot (WB)
          VEGF-165 Antibody in Western Blot (WB)
          VEGF-165 Antibody in ELISA (ELISA)
          VEGF-165 Polyclonal Antibody, Biotin, PeproTech®

          Product Details

          500-P10GBT-25UG

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.1-0.2 µg/mL
          -

          ELISA (ELISA)

          0.25-1.0 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Goat

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          E.coli-derived, 38.2 kDa Recombinant Human VEGF 165
          View immunogen

          Conjugate

          Biotin Biotin Biotin

          Form

          Lyophilized

          Concentration

          0.1-1.0 mg/mL

          Amount

          25 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS

          Contains

          no preservative

          Storage conditions

          -20°C

          Shipping conditions

          Ambient

          RRID

          AB_2929247

          Product Specific Information

          AA Sequence of recombinant protein: APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR.

          Preparation: Produced from sera of goats immunized with highly pure Recombinant Human VEGF 165. Anti-Human VEGF 165-specific antibody was purified by affinity chromatography and then biotinylated.

          Sandwich ELISA: To detect Human VEGF 165 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human VEGF 165 (500-P10G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human VEGF 165.

          Western Blot: To detect hVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hVEGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

          500-P10GBT-1MG will be provided as 2 x 500 µg

          Target Information

          The vascular endothelial growth factor (VEGF) family currently includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system have been shown to be the fundamental regulators in the cell signaling of angiogenesis. Most tumors have the absolute requirement of angiogenesis and VEGF has been described as the most potent angiogenic cytokine linked to this process. To date 5 different isoforms of VEGF have been described. These isoforms are generated as the result of alternative splicing from a single VEGF gene. These various isoforms have been shown to bind to two tyrosine-kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which have been found to be expressed almost exclusively on endothelial cells.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: growth factor; H-VEGF-165; L-VEGF; M-VEGF-165; vascular endothelial growth factor; Vascular endothelial growth factor A, long form; vascular endothelial growth factor A121; vascular endothelial growth factor A165; Vascular permeability factor; vascular permeability factor precursor; VEGF121; VEGF165; VPF

          View more View less

          Gene Aliases: L-VEGF; MVCD1; VEGF; VEGFA; VPF

          View more View less

          UniProt ID: (Human) P15692

          View more View less

          Entrez Gene ID: (Human) 7422

          View more View less

          Function(s)
          fibronectin binding cytokine activity platelet-derived growth factor receptor binding vascular endothelial growth factor receptor binding protein binding growth factor activity heparin binding transmembrane receptor protein tyrosine kinase activator activity neuropilin binding chemoattractant activity identical protein binding protein homodimerization activity vascular endothelial growth factor receptor 1 binding vascular endothelial growth factor receptor 2 binding receptor agonist activity extracellular matrix binding
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter angiogenesis ovarian follicle development blood vessel development patterning of blood vessels vasculogenesis response to hypoxia in utero embryonic development kidney development positive regulation of protein phosphorylation endothelial cell proliferation positive regulation of endothelial cell proliferation vasculature development lymphangiogenesis sprouting angiogenesis cell migration involved in sprouting angiogenesis positive regulation of neuroblast proliferation epithelial cell maturation positive regulation of receptor internalization immune system process basophil chemotaxis positive regulation of leukocyte migration heart morphogenesis outflow tract morphogenesis coronary vein morphogenesis regulation of transcription from RNA polymerase II promoter apoptotic process phospholipase C-activating G-protein coupled receptor signaling pathway nervous system development neuroblast proliferation mesoderm development lactation cell proliferation positive regulation of cell proliferation regulation of cell shape positive regulation of endothelial cell migration positive regulation of gene expression negative regulation of gene expression negative regulation of epithelial to mesenchymal transition regulation of nitric oxide mediated signal transduction cell migration negative regulation of cell-cell adhesion cell differentiation monocyte differentiation macrophage differentiation lung development positive regulation of cell migration epithelial cell differentiation post-embryonic camera-type eye development retinal ganglion cell axon guidance positive regulation of protein complex assembly positive regulation of protein autophosphorylation tube formation endothelial cell chemotaxis cellular response to vascular endothelial growth factor stimulus lymph vessel morphogenesis vascular endothelial growth factor receptor-2 signaling pathway positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway vascular endothelial growth factor signaling pathway positive regulation of cell proliferation by VEGF-activated platelet derived growth factor receptor signaling pathway VEGF-activated neuropilin signaling pathway regulation of growth vasodilation eye photoreceptor cell development negative regulation of apoptotic process positive regulation of vascular permeability positive regulation of I-kappaB kinase/NF-kappaB signaling surfactant homeostasis positive regulation of MAPK cascade positive regulation of blood vessel endothelial cell migration endothelial cell migration negative regulation of fat cell differentiation regulation of endothelial cell differentiation positive regulation of osteoblast differentiation positive regulation of angiogenesis positive regulation of cell adhesion positive regulation of transcription from RNA polymerase II promoter vascular endothelial growth factor receptor signaling pathway cell maturation blood vessel morphogenesis camera-type eye morphogenesis positive regulation of axon extension involved in axon guidance artery morphogenesis homeostasis of number of cells within a tissue epithelial cell proliferation positive regulation of epithelial cell proliferation positive regulation of peptidyl-tyrosine phosphorylation positive chemotaxis positive regulation of positive chemotaxis induction of positive chemotaxis positive regulation of cell division positive regulation of focal adhesion assembly positive regulation of protein kinase B signaling cardiac muscle cell development primitive erythrocyte differentiation bone trabecula formation lung vasculature development mammary gland alveolus development positive regulation of mast cell chemotaxis cardiac vascular smooth muscle cell development coronary artery morphogenesis vascular wound healing bone trabecula morphogenesis positive regulation of ERK1 and ERK2 cascade cellular response to hypoxia dopaminergic neuron differentiation commissural neuron axon guidance positive regulation of cell migration involved in sprouting angiogenesis positive regulation of branching involved in ureteric bud morphogenesis motor neuron migration cellular stress response to acid chemical cell-cell adhesion positive regulation of cold-induced thermogenesis positive regulation of vascular endothelial growth factor signaling pathway positive regulation of trophoblast cell migration positive regulation of lymphangiogenesis regulation of hematopoietic progenitor cell differentiation negative regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of protein localization to early endosome negative regulation of establishment of endothelial barrier negative regulation of adherens junction organization positive regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis positive regulation of sprouting angiogenesis positive regulation of epithelial tube formation positive regulation of blood vessel branching negative regulation of blood-brain barrier permeability negative regulation of cell-cell adhesion mediated by cadherin positive regulation of DNA biosynthetic process positive regulation of endothelial cell chemotaxis
          It has to be done as per old AB suggested Products section.

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