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          • Primary Antibodies ›
          • IGF1 Antibodies

          Invitrogen

          IGF1 Polyclonal Antibody, PeproTech®

          9 Published Figures
          7 References
          View all (40) IGF1 antibodies

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          Cite IGF1 Polyclonal Antibody, PeproTech®

          Additional Information:
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          • Antibody Testing Data (3)
          • Published Figures (9)
          IGF1 Antibody in Western Blot (WB)
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          IGF1 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 12

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          IGF1 Antibody (500-P11-100UG) in WB

          Western Blot: To detect Human IGF-I by Western Blot analysis IGF1 Polyclonal Antibody (Product # 500-P11-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IGF-I is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          IGF1 Antibody in Western Blot (WB)
          IGF1 Antibody in Western Blot (WB)
          IGF1 Antibody in ELISA (ELISA)
          IGF1 Antibody in Western Blot (WB)
          IGF1 Antibody in Western Blot (WB)
          IGF1 Antibody in Western Blot (WB)
          IGF1 Antibody in Western Blot (WB)
          IGF1 Antibody in Western Blot (WB)
          IGF1 Antibody in Western Blot (WB)
          IGF1 Antibody in Western Blot (WB)
          IGF1 Antibody in Western Blot (WB)
          IGF1 Antibody in Western Blot (WB)
          IGF1 Polyclonal Antibody, PeproTech®

          Product Details

          500-P11-100UG

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.1-0.2 µg/mL
          View 2 publications 2 publications

          ELISA (ELISA)

          0.5-2.0 µg/mL
          -

          Neutralization (Neu)

          -
          View 1 publication 1 publication

          In vitro Assay (In vitro)

          -
          View 1 publication 1 publication

          Immunostaining (IS)

          -
          View 3 publications 3 publications
          Product Specifications

          Species Reactivity

          Human

          Published species

          Human, Mouse, Rat

          Host/Isotype

          Rabbit

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          E.coli-derived Recombinant Human IGF-I
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Lyophilized

          Concentration

          0.1-1.0 mg/mL

          Amount

          100 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS

          Contains

          no preservative

          Storage conditions

          -20°C

          Shipping conditions

          Ambient

          RRID

          AB_2929255

          Product Specific Information

          AA Sequence of recombinant protein: GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA.

          Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IGF-I. Anti-Human IGF-I-specific antibody was purified by affinity chromatography employing an immobilized Human IGF-I matrix.

          Sandwich ELISA: To detect Human IGF-I by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IGF-I (500-P11Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IGF-I.

          Western Blot: To detect Human IGF-I by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IGF-I is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

          Target Information

          IGF1 (Insulin-like growth factor-1) is structurally and functionally related to insulin but has a much higher growth-promoting activity. A variety of cellular responses are induced by IGF1, including cell proliferation, differentiation, migration, and survival. Further, IGF1 is a polypeptide growth factor that stimulates the proliferation of a wide range of cell types in muscle, bone, and cartilage tissue. IGF1 stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regards to enhancing glucose uptake. In circulation, IGFs are predominantly bound to binding proteins (IGFBPs) which prolong the half-life of the IGFs and play a role in delivering them to target tissues. IGF-I is known as one of the most potent activators of the AKT signaling pathway which is known to be a stimulator of proliferation and an inhibitor of programmed cell death. Moreover, mature human IGF-I is 100% homologous with bovine and porcine proteins. Low levels of IGF1 have been linked to Alzheimer's disease. IGF1 is processed from a precursor, bound by a specific receptor, and secreted. Defects in the IGF1 gene are a cause of insulin-like growth factor 1 deficiency and several transcript variants encoding different isoforms have been found.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: H-IGF-1; IGF; IGF-I; IGF-IA; IGF-IB; IGF1; IGF1A; IGFIa; Insulin like growth factor; Insulin-like growth factor 1; insulin-like growth factor 1 (somatomedin C); Insulin-like growth factor I; insulin-like growth factor I precursor; insulin-like growth factor IB; insulin-like growth factor IB prepropeptide; insulin-like growth factor precursor; M-IGF-1; Mechano growth factor; MGF; OTTHUMP00000195084; R-IGF-1; Somatomedin C; Somatomedin-C; unnamed protein product

          View more View less

          Gene Aliases: IBP1; IGF; IGF-1; IGF-I; IGF1; IGFI; MGF

          View more View less

          UniProt ID: (Human) P05019

          View more View less

          Entrez Gene ID: (Human) 3479

          View more View less

          Function(s)
          insulin receptor binding insulin-like growth factor receptor binding integrin binding hormone activity protein binding growth factor activity protein kinase activator activity transmembrane receptor protein tyrosine kinase activator activity receptor agonist activity
          Process(es)
          skeletal system development osteoblast differentiation cell activation epithelial to mesenchymal transition blood vessel remodeling signal transduction Ras protein signal transduction nervous system development muscle organ development circadian rhythm cell proliferation positive regulation of cell proliferation negative regulation of cell proliferation insulin receptor signaling pathway response to heat glycolate metabolic process glial cell differentiation regulation of gene expression positive regulation of glycoprotein biosynthetic process positive regulation of cardiac muscle hypertrophy positive regulation of gene expression negative regulation of gene expression skeletal muscle satellite cell maintenance involved in skeletal muscle regeneration muscle hypertrophy myotube differentiation myotube cell development positive regulation of smooth muscle cell migration cerebellar granule cell precursor proliferation positive regulation of cerebellar granule cell precursor proliferation proteoglycan biosynthetic process lung development positive regulation of cell migration androgen receptor signaling pathway mammary gland development exocrine pancreas development positive regulation of myelination activation of protein kinase B activity negative regulation of interleukin-1 beta production negative regulation of tumor necrosis factor production regulation of establishment or maintenance of cell polarity negative regulation of smooth muscle cell apoptotic process multicellular organism growth bone mineralization involved in bone maturation wound healing positive regulation of activated T cell proliferation negative regulation of apoptotic process positive regulation of DNA binding positive regulation of MAPK cascade protein kinase B signaling positive regulation of insulin-like growth factor receptor signaling pathway type B pancreatic cell proliferation regulation of nitric oxide biosynthetic process myoblast differentiation positive regulation of osteoblast differentiation positive regulation of glycogen biosynthetic process positive regulation of glycolytic process positive regulation of mitotic nuclear division positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of glucose import positive regulation of Ras protein signal transduction insulin-like growth factor receptor signaling pathway positive regulation of fibroblast proliferation lung alveolus development cell development positive regulation of smooth muscle cell proliferation branching morphogenesis of an epithelial tube inner ear development chondroitin sulfate proteoglycan biosynthetic process positive regulation of epithelial cell proliferation positive regulation of protein secretion protein stabilization regulation of protein metabolic process myoblast proliferation positive regulation of protein kinase B signaling negative regulation of oocyte development growth hormone receptor signaling pathway lung vasculature development lung lobe morphogenesis Type I pneumocyte differentiation Type II pneumocyte differentiation prostate epithelial cord arborization involved in prostate glandular acinus morphogenesis prostate gland growth prostate gland epithelium morphogenesis prostate gland stromal morphogenesis negative regulation of androgen receptor signaling pathway positive regulation of cell growth involved in cardiac muscle cell development negative regulation of ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade positive regulation of calcineurin-NFAT signaling cascade cellular response to glucose stimulus negative regulation of release of cytochrome c from mitochondria extrinsic apoptotic signaling pathway in absence of ligand receptor signaling pathway via STAT postsynaptic modulation of chemical synaptic transmission negative regulation of neuroinflammatory response negative regulation of beta-amyloid formation positive regulation of trophectodermal cell proliferation cellular response to beta-amyloid positive regulation of type B pancreatic cell proliferation positive regulation of vascular smooth muscle cell proliferation negative regulation of vascular associated smooth muscle cell apoptotic process cellular response to insulin-like growth factor stimulus positive regulation of myoblast proliferation positive regulation of transcription regulatory region DNA binding negative regulation of apoptotic signaling pathway negative regulation of extrinsic apoptotic signaling pathway
          It has to be done as per old AB suggested Products section.

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