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Invitrogen

IGF1 Polyclonal Antibody, PeproTech®

9 Published Figures
7 References
View all (43) IGF1 antibodies

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IGF1 Polyclonal Antibody, PeproTech®

Product Details

500-P11-100UG

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.2 µg/mL
View 2 publications 2 publications

ELISA (ELISA)

0.5-2.0 µg/mL
-

Neutralization (Neu)

-
View 1 publication 1 publication

In vitro Assay (In vitro)

-
View 1 publication 1 publication

Immunostaining (IS)

-
View 3 publications 3 publications
Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human IGF-I
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Amount

100 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2929255

Product Specific Information

AA Sequence of recombinant protein: GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IGF-I. Anti-Human IGF-I-specific antibody was purified by affinity chromatography employing an immobilized Human IGF-I matrix.

Sandwich ELISA: To detect Human IGF-I by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IGF-I (500-P11Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IGF-I.

Western Blot: To detect Human IGF-I by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IGF-I is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

IGF1 (Insulin-like growth factor-1) is structurally and functionally related to insulin but has a much higher growth-promoting activity. A variety of cellular responses are induced by IGF1, including cell proliferation, differentiation, migration, and survival. Further, IGF1 is a polypeptide growth factor that stimulates the proliferation of a wide range of cell types in muscle, bone, and cartilage tissue. IGF1 stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regards to enhancing glucose uptake. In circulation, IGFs are predominantly bound to binding proteins (IGFBPs) which prolong the half-life of the IGFs and play a role in delivering them to target tissues. IGF-I is known as one of the most potent activators of the AKT signaling pathway which is known to be a stimulator of proliferation and an inhibitor of programmed cell death. Moreover, mature human IGF-I is 100% homologous with bovine and porcine proteins. Low levels of IGF1 have been linked to Alzheimer's disease. IGF1 is processed from a precursor, bound by a specific receptor, and secreted. Defects in the IGF1 gene are a cause of insulin-like growth factor 1 deficiency and several transcript variants encoding different isoforms have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: H-IGF-1; IGF; IGF-IA; IGF-IB; IGF1; IGF1A; IGFIa; Insulin like growth factor; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor I; insulin-like growth factor I precursor; insulin-like growth factor IB; insulin-like growth factor IB prepropeptide; insulin-like growth factor precursor; M-IGF-1; mechano growth factor; OTTHUMP00000195084; R-IGF-1; Somatomedin C; somatomedin-C; unnamed protein product

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Gene Aliases: IGF; IGF-I; IGFI; MGF

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UniProt ID: (Human) P05019

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Entrez Gene ID: (Human) 3479

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Function(s)
insulin receptor binding insulin-like growth factor receptor binding integrin binding hormone activity protein binding growth factor activity receptor agonist activity
Process(es)
skeletal system development osteoblast differentiation cell activation epithelial to mesenchymal transition signal transduction Ras protein signal transduction muscle organ development circadian rhythm cell proliferation positive regulation of cell proliferation insulin receptor signaling pathway response to heat glycolate metabolic process regulation of gene expression positive regulation of glycoprotein biosynthetic process positive regulation of cardiac muscle hypertrophy positive regulation of gene expression negative regulation of gene expression skeletal muscle satellite cell maintenance involved in skeletal muscle regeneration muscle hypertrophy myotube cell development positive regulation of smooth muscle cell migration proteoglycan biosynthetic process positive regulation of cell migration positive regulation of myelination activation of protein kinase B activity negative regulation of interleukin-1 beta production negative regulation of tumor necrosis factor production negative regulation of smooth muscle cell apoptotic process bone mineralization involved in bone maturation wound healing positive regulation of activated T cell proliferation negative regulation of apoptotic process positive regulation of DNA binding positive regulation of MAPK cascade positive regulation of skeletal muscle tissue regeneration positive regulation of insulin-like growth factor receptor signaling pathway myoblast differentiation positive regulation of osteoblast differentiation positive regulation of glycogen biosynthetic process positive regulation of glycolytic process positive regulation of mitotic nuclear division positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of glucose import positive regulation of Ras protein signal transduction insulin-like growth factor receptor signaling pathway positive regulation of fibroblast proliferation positive regulation of smooth muscle cell proliferation positive regulation of epithelial cell proliferation positive regulation of protein secretion protein stabilization myoblast proliferation positive regulation of protein kinase B signaling negative regulation of oocyte development growth hormone receptor signaling pathway positive regulation of cell growth involved in cardiac muscle cell development positive regulation of ERK1 and ERK2 cascade positive regulation of calcineurin-NFAT signaling cascade negative regulation of release of cytochrome c from mitochondria receptor signaling pathway via STAT postsynaptic modulation of chemical synaptic transmission negative regulation of neuroinflammatory response negative regulation of beta-amyloid formation positive regulation of trophectodermal cell proliferation cellular response to beta-amyloid positive regulation of vascular smooth muscle cell proliferation negative regulation of vascular associated smooth muscle cell apoptotic process positive regulation of transcription regulatory region DNA binding negative regulation of apoptotic signaling pathway negative regulation of extrinsic apoptotic signaling pathway
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