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Invitrogen
IL-2 Polyclonal Antibody, PeproTech®
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FIGURE: 1 / 3
IL-2 Antibody (500-P111-50UG) in WB
Western Blot: To detect Murine IL-2 by Western Blot analysis IL-2 Polyclonal Antibody (Product # 500-P111-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine IL-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
500-P111-50UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Murine IL-2
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: PTSSSTSSST AEAQQQQQQQ QQQQQHLEQL LMDLQELLSR MENYRNLKLP RMLTFKFYLP KQATELKDLQ CLEDELGPLR HVLDLTQSKS FQLEDAENFI SNIRVTVVKL KGSDNTFECQ FDDESATVVD FLRRWIAFCQ SIISTSPQ.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-2. Anti-Murine IL-2-specific antibody was purified by affinity chromatography employing an immobilized Murine IL-2 matrix.
Sandwich ELISA: To detect Murine IL-2 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine IL-2 (500-P111Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine IL-2.
Western Blot: To detect Murine IL-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine IL-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: H-IL-2; il 2; IL2; ILN; Interleukin; interleukin 2 prepeptide; interleukin-2; Interleukin2; Involved in regulation of T cell clonal expansion; M-IL-2; T-cell growth factor; T-cell growth factor (TCGF); TCGF
Gene Aliases: Il-2
UniProt ID: (Mouse) P04351
Entrez Gene ID: (Mouse) 16183
negative regulation of protein phosphorylation
positive regulation of protein phosphorylation
leukocyte activation involved in immune response
positive regulation of immunoglobulin production
negative regulation of B cell apoptotic process
immune response
G-protein coupled receptor signaling pathway
phospholipase C-activating G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
gene expression
positive regulation of B cell proliferation
positive regulation of interferon-gamma production
positive regulation of interleukin-17 production
interleukin-2-mediated signaling pathway
positive regulation of T cell proliferation
positive regulation of activated T cell proliferation
positive regulation of tyrosine phosphorylation of STAT protein
response to ethanol
positive regulation of T cell differentiation
positive regulation of regulatory T cell differentiation
positive regulation of transcription from RNA polymerase II promoter
regulation of T cell homeostatic proliferation
positive regulation of isotype switching to IgG isotypes
negative regulation of lymphocyte proliferation
negative regulation of inflammatory response
positive regulation of dendritic spine development
extrinsic apoptotic signaling pathway in absence of ligand
receptor signaling pathway via STAT
positive regulation of plasma cell differentiation
negative regulation of T-helper 17 cell differentiation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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