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Invitrogen
DEFA1 Polyclonal Antibody, PeproTech®
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FIGURE: 1 / 3
DEFA1 Antibody (500-P126G-50UG) in WB
Western Blot: To detect Human NP-1 by Western Blot analysis DEFA1 Polyclonal Antibody (Product # 500-P126G-1MG) can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human NP-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
500-P126G-50UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human NP-1
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
0.1-1.0 mg/mL once reconstituted
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: ACYCRIPACI AGERRYGTCI YQGRLWAFCC.
Preparation: Produced from sera of goats immunized with highly pure Recombinant Human NP-1. Anti-Human NP-1-specific antibody was purified by affinity chromatography employing an immobilized Human NP-1 matrix.
Sandwich ELISA: To detect Human NP-1 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human NP-1 (500-P126GBt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human NP-1.
Western Blot: To detect Human NP-1 by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human NP-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
Defensins are a family of microbicidal and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 3 by only one amino acid. This gene and the gene encoding defensin, alpha 3 are both subject to copy number variation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: defensin 1; defensin, alpha 1, myeloid-related sequence; defensin, alpha 2; myeloid-related sequence; neutrophil peptide 1 precursor; unnamed protein product
Gene Aliases: DEF1; DEFA2; HNP-1; HP-1; HP1; MRS
UniProt ID: (Human) P59665
Entrez Gene ID: (Human) 1667
innate immune response in mucosa
chemotaxis
defense response
immune response
T cell chemotaxis
antibacterial humoral response
intracellular estrogen receptor signaling pathway
killing of cells of other organism
innate immune response
defense response to Gram-negative bacterium
defense response to Gram-positive bacterium
membrane disruption in other organism
antimicrobial humoral immune response mediated by antimicrobial peptide
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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