Search

  • Statut des commandes
  • Commande rapide
  • Promos

Invitrogen

RANKL (soluble) Polyclonal Antibody, PeproTech®

7 References
View all (79) RANKL (RANK Ligand) antibodies

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Additional Information:
{{banner.description}}
{{$ctrl.isSkuNotesCollapsed ? 'View more' : 'View less'}}
RANKL (soluble) Polyclonal Antibody, PeproTech®

Product Details

500-P133-100UG

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.2 µg/mL
View 2 publications 2 publications

ELISA (ELISA)

0.5-2.0 µg/mL
View 2 publications 2 publications

In vitro Assay (In vitro)

-
View 2 publications 2 publications

Immunostaining (IS)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human sRANK Ligand
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Amount

100 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2929401

Product Specific Information

AA Sequence of recombinant protein: MEKAMVDGSW LDLAKRSKLE AQPFAHLTIN ATDIPSGSHK VSLSSWYHDR GWAKISNMTF SNGKLIVNQD GFYYLYANIC FRHHETSGDL ATEYLQLMVY VTKTSIKIPS SHTLMKGGST KYWSGNSEFH FYSINVGGFF KLRSGEEISI EVSNPSLLDP DQDATYFGAF KVRDID.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human sRANK Ligand. Anti-Human sRANK Ligand-specific antibody was purified by affinity chromatography employing an immobilized Human sRANK Ligand matrix.

Sandwich ELISA: To detect Human sRANK Ligand by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human sRANK Ligand (500-P133Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human sRANK Ligand.

Western Blot: To detect Human sRANK Ligand by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human sRANK Ligand is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CD254; H-RANK-L; M-Rank-L; ODF; OPGL; Osteoclast differentiation factor; Osteoprotegerin ligand; rank-l; RANKL; receptor activator of nuclear factor kappa B ligand; Receptor activator of nuclear factor kappa-B ligand; RP11-86N24.2; srankl; TNF-related activation-induced cytokine; TNF-related ligand; TRANCE; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand 6B; Tumor necrosis factor ligand superfamily member 11; tumor necrosis factor superfamily member 11

View more View less

Gene Aliases: CD254; hRANKL2; ODF; OPGL; OPTB2; RANKL; sOdf; TNFSF11; TNLG6B; TRANCE

View more View less

UniProt ID: (Human) O14788

View more View less

Entrez Gene ID: (Human) 8600

View more View less

Function(s)
receptor binding cytokine activity tumor necrosis factor receptor binding protein binding tumor necrosis factor receptor superfamily binding identical protein binding receptor agonist activity
Process(es)
negative regulation of transcription from RNA polymerase II promoter ossification osteoclast proliferation monocyte chemotaxis immune response cell communication cell surface receptor signaling pathway JNK cascade organ morphogenesis positive regulation of gene expression cytokine-mediated signaling pathway calcium-mediated signaling signaling cell differentiation osteoclast differentiation tumor necrosis factor-mediated signaling pathway mammary gland epithelial cell proliferation positive regulation of homotypic cell-cell adhesion osteoclast development paracrine signaling positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of MAPK cascade protein kinase B signaling tooth eruption bone resorption regulation of osteoclast differentiation positive regulation of osteoclast differentiation positive regulation of bone resorption positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade positive regulation of T cell activation positive regulation of corticotropin-releasing hormone secretion positive regulation of protein kinase B signaling calcium ion homeostasis bone development mammary gland alveolus development positive regulation of ERK1 and ERK2 cascade positive regulation of fever generation by positive regulation of prostaglandin secretion positive regulation of NIK/NF-kappaB signaling positive regulation of intracellular signal transduction cellular response to leukemia inhibitory factor positive regulation of osteoclast development positive regulation of extrinsic apoptotic signaling pathway
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support