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Invitrogen
RANK (soluble) Polyclonal Antibody, Biotin, PeproTech®
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FIGURE: 1 / 3
RANK (soluble) Antibody (500-P144BT-50UG) in WB
Western Blot: To detect hsRANK Receptor by Western Blot analysis RANK (soluble) Polyclonal Antibody, Biotin (Product # 500-P144BT-500UG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hsRANK Receptor is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P144BT-50UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived, 19.3 kDa Recombinant Human sRANK Receptor
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: MQIAPPCTSE KHYEHLGRCC NKCEPGKYMS SKCTTTSDSV CLPCGPDEYL DSWNEEDKCL LHKVCDTGKA LVAVVAGNST TPRRCACTAG YHWSQDCECC RRNTECAPGL GAQHPLQLNK DTVCKPCLAG YFSDAFSSTD KCRPWTNCTF LGKRVEHHGT EKSDAVCSSS LPARK.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human sRANK Receptor. Anti-Human sRANK Receptor-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human sRANK Receptor by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human sRANK Receptor (500-P144) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human sRANK Receptor.
Western Blot: To detect hsRANK Receptor by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hsRANK Receptor is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P144BT-1MG will be provided as 2 x 500 µg
Target Information
RANK is a member of the tumor necrosis factor receptor (TNFR) family. The full length mouse RANK cDNA encodes a type I transmembrane protein with an extracellular and a cytoplasmic domain. The extracellular domain contains two potential N-linked glycosylation sites. RANK shares significant amino acid homology with other members of the TNFR family in its extracellular four cysteine-rich repeats. Human and murine RANK share 81% amino acid identity in their extracellular domains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD265; familial expansile osteolysis; loss of heterozygosity, 18, chromosomal region 1; ODFR; Osteoclast differentiation factor receptor; Paget disease of bone 2; Receptor activator of NF-KB; receptor activator of nuclear factor-kappa B; TRANCE receptor; Tumor necrosis factor receptor superfamily member 11A; tumor necrosis factor receptor superfamily, member 11a, NFKB activator
Gene Aliases: CD265; FEO; LOH18CR1; ODFR; OFE; OPTB7; OSTS; PDB2; RANK; TNFRSF11A; TRANCE-R; TRANCER
UniProt ID: (Human) Q9Y6Q6
Entrez Gene ID: (Human) 8792
ossification
adaptive immune response
immune system process
monocyte chemotaxis
signal transduction
cell-cell signaling
positive regulation of cell proliferation
response to mechanical stimulus
osteoclast differentiation
response to lipopolysaccharide
response to insulin
tumor necrosis factor-mediated signaling pathway
cellular response to zinc ion starvation
response to tumor necrosis factor
positive regulation of I-kappaB kinase/NF-kappaB signaling
response to ethanol
positive regulation of osteoclast differentiation
positive regulation of bone resorption
positive regulation of JNK cascade
lymph node development
circadian temperature homeostasis
mammary gland alveolus development
positive regulation of ERK1 and ERK2 cascade
response to interleukin-1
positive regulation of fever generation by positive regulation of prostaglandin secretion
multinuclear osteoclast differentiation
positive regulation of NIK/NF-kappaB signaling
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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