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Invitrogen
CXCL6 Polyclonal Antibody, Biotin, PeproTech®
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FIGURE: 1 / 1
CXCL6 Antibody (500-P146BT-500UG) in ELISA
Sandwich ELISA: To detect Murine LIX (CXCL6) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of CXCL6 Polyclonal Antibody, Biotin (Product # 500-P146BT-500UG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech CXCL6 Polyclonal Antibody (Product # 500-P146-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine LIX (CXCL6).
Product Details
500-P146BT-500UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Murine LIX (CXCL6) (92 a.a.)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Amount
500 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: APSSVIAATE LRCVCLTVTP KINPKLIANL EVIPAGPQCP TVEVIAKLKN QKEVCLDPEA PVIKKIIQKI LGSDKKKAKR NALAVERTAS VQ.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Muine LIX (CXCL6). Anti-Murine LIX (CXCL6)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Murine LIX (CXCL6) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine LIX (CXCL6) (500-P146) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine LIX (CXCL6).
Western Blot: To detect mLIX by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mLIX is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P146BT-1MG will be provided as 2 x 500 µg
Target Information
CXCL6 is a chemotactic for neutrophil granulocytes. It signals through binding and activation of its receptors (CXCR1 and CXCR2). In addition to its chemotactic and angiogenic properties, it has strong antibacterial activity against Gram-positive and Gram-negative bacteria (90-fold-higher when compared to CXCL5 and CXCL7).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C Cmotif chemokine; C X C motif chemokine; CC motif chemokine; CCmotif chemokine; CKA3; CXC; CXC motif chemokine; CXCL
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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