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Invitrogen
FGF10 Polyclonal Antibody, PeproTech®
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FIGURE: 1 / 2
FGF10 Antibody (500-P151G-100UG) in WB
Western Blot: To detect Human FGF-10 by Western Blot analysis FGF10 Polyclonal Antibody (Product # 500-P151G-1MG) can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human FGF-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
500-P151G-100UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human FGF-10
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: MLGQDMVSPE ATNSSSSSFS SPSSAGRHVR SYNHLQGDVR WRKLFSFTKY FLKIEKNGKV SGTKKENCPY SILEITSVEI GVVAVKAINS NYYLAMNKKG KLYGSKEFNN DCKLKERIEE NGYNTYASFN WQHNGRQMYV ALNGKGAPRR GQKTRRKNTS AHFLPMVVHS.
Preparation: Produced from sera of goats immunized with highly pure Recombinant Human FGF-10. Anti-Human FGF-10-specific antibody was purified by affinity chromatography employing an immobilized Human FGF-10 matrix.
Sandwich ELISA: To detect Human FGF-10 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human FGF-10 (500-P151GBt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human FGF-10.
Western Blot: To detect Human FGF-10 by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human FGF-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
FGF10 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. Studies of the mouse homolog of suggested that this gene is required for embryonic epidermal morphogenesis including brain development, lung morphogenesis, and initiation of lim bud formation. This protein is also implicated to be a primary factor in the process of wound healing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FGF; FGF-10; FGF10; Fibroblast growth factor; fibroblast growth factor 10; FGF10; keratinocyte growth factor 2; keratinocyte growth factor-2; KGF2; produced by fibroblasts of urinary bladder lamina propria
Gene Aliases: LADD3
UniProt ID: (Human) O15520
Entrez Gene ID: (Human) 2255
angiogenesis
metanephros development
mesonephros development
salivary gland development
positive regulation of cell proliferation
fibroblast growth factor receptor signaling pathway
positive regulation of epithelial cell migration
positive regulation of keratinocyte proliferation
neurogenesis
actin cytoskeleton organization
cell differentiation
regulation of cell migration
response to estradiol
response to lipopolysaccharide
positive regulation of ATPase activity
lacrimal gland development
protein localization to cell surface
wound healing
tissue regeneration
positive regulation of MAPK cascade
positive regulation of DNA repair
positive regulation of transcription, DNA-templated
positive regulation of Ras protein signal transduction
regulation of saliva secretion
thymus development
branching morphogenesis of an epithelial tube
positive regulation of lymphocyte proliferation
epithelial cell proliferation
urothelial cell proliferation
positive regulation of urothelial cell proliferation
positive regulation of epithelial cell proliferation
positive regulation of peptidyl-tyrosine phosphorylation
positive chemotaxis
positive regulation of keratinocyte migration
radial glial cell differentiation
positive regulation of epithelial cell proliferation involved in wound healing
lung epithelium development
lung saccule development
bud outgrowth involved in lung branching
secretion by lung epithelial cell involved in lung growth
tear secretion
ERK1 and ERK2 cascade
positive regulation of hair follicle cell proliferation
positive regulation of G1/S transition of mitotic cell cycle
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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