Search
Invitrogen
beta-2 Defensin Polyclonal Antibody, Biotin, PeproTech®
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 3
beta-2 Defensin Antibody (500-P161GBT-25UG) in WB
Western Blot: To detect hBD-2 by Western Blot analysis beta-2 Defensin Polyclonal Antibody, Biotin (Product # 500-P161GBT-500UG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hBD-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P161GBT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Goat
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human BD-2
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Amount
25 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: GIGDPVTCLK SGAICHPVFC PRRYKQIGTC GLPGTKCCKK P.
Preparation: Produced from sera of goats immunized with highly pure Recombinant Human BD-2. Anti-Human BD-2-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hBD-2 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human BD-2 (500-P161G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hBD-2.
Western Blot: To detect hBD-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hBD-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P161GBT-1MG will be provided as 2 x 500 µg
Target Information
Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The alpha-defensins are distinguished from the beta-defensins by the pairing of their three disulfide bonds. To date, six human beta-defensins have been identified; BD-1, BD-2, BD-3, BD-4, BD-5 and BD-6. beta-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they can act as chemoattractants towards immature dendritic cells and memory T cells. The beta-defensin proteins are expressed as the C-terminal portion of precursors, and are released by proteolytic cleavage of a signal sequence and, in some cases, a propeptide sequence. Beta-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: BD-2; beta defensin-2; Beta-defensin 2; beta-defensin 4A; Defensin beta 2; Defensin beta 4A; Defensin beta2; Defensin, beta 2; defensin, beta 4; Defensin-beta2; SAP1; Skin-antimicrobial peptide 1
Gene Aliases: BD-2; DEFB-2; DEFB102; DEFB2; DEFB4; DEFB4A; DEFB4B; HBD-2; SAP1
UniProt ID: (Human) O15263
Entrez Gene ID: (Human) 1673
chemotaxis
defense response
immune response
G-protein coupled receptor signaling pathway
killing of cells of other organism
defense response to bacterium
defense response to Gram-negative bacterium
defense response to Gram-positive bacterium
defense response to fungus
positive chemotaxis
cell chemotaxis
antifungal innate immune response
antimicrobial humoral immune response mediated by antimicrobial peptide
defense response to symbiont
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support