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TNFR2 (soluble) Polyclonal Antibody, PeproTech®
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FIGURE: 1 / 3
TNFR2 (soluble) Antibody (500-P168-100UG) in WB
Western Blot: To detect Human sTNF Receptor Type II by Western Blot analysis TNFR2 (soluble) Polyclonal Antibody (Product # 500-P168-1MG) can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human sTNF Receptor Type II is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P168-100UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human sTNF Receptor Type II
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: MAPEPGSTCR LREYYDQTAQ MCCSKCSPGQ HAKVFCTKTS DTVCDSCEDS TYTQLWNWVP ECLSCGSRCS SDQVETQACT REQNRICTCR PGWYCALSKQ EGCRLCAPLR KCRPGFGVAR PGTETSDVVC KPCAPGTFSN TTSSTDICRP HQICNVVAIP GNASMDAVCT STSP.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human sTNF Receptor Type II. Anti-Human sTNF Receptor Type II-specific antibody was purified by affinity chromatography employing an immobilized Human sTNF Receptor Type II matrix.
Sandwich ELISA: To detect Human sTNF Receptor Type II by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human sTNF Receptor Type II (500-P168Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human sTNF Receptor Type II.
Western Blot: To detect Human sTNF Receptor Type II by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human sTNF Receptor Type II is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
TNFR2 (tumor necrosis factor receptor superfamily member 1B) is a receptor with high affinity for TNFSF2/TNF-alpha. It has a much lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. TNFR2 mediates most of the metabolic effects of TNF-alpha.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 75-kda; CD120b; p75; p75 TNF receptor; p80 TNF-alpha receptor; sCD120b; soluble CD120b; soluble TNFR2; sTNF R2; sTNFR 2; sTNFR2; TNF-R2; TNF-RII; TNFR; TNFR II; tumor necrosis factor beta receptor; tumor necrosis factor binding protein 2; Tumor necrosis factor receptor; Tumor necrosis factor receptor 2; tumor necrosis factor receptor protein (TNFR); Tumor necrosis factor receptor superfamily member 1B; Tumor necrosis factor receptor type II
Gene Aliases: CD120b; p75; p75TNFR; TBPII; TNF-R-II; TNF-R75; TNFBR; TNFR1B; TNFR2; TNFR80; TNFRSF1B
UniProt ID: (Human) P20333
Entrez Gene ID: (Human) 7133
regulation of cytokine production involved in immune response
regulation of T cell cytokine production
aortic valve development
pulmonary valve development
negative regulation of extracellular matrix constituent secretion
apoptotic process
inflammatory response
cell surface receptor signaling pathway
intrinsic apoptotic signaling pathway in response to DNA damage
negative regulation of cardiac muscle hypertrophy
regulation of myelination
positive regulation of myelination
tumor necrosis factor-mediated signaling pathway
regulation of T cell proliferation
positive regulation of oligodendrocyte differentiation
negative regulation of inflammatory response
RNA destabilization
positive regulation of membrane protein ectodomain proteolysis
cellular response to lipopolysaccharide
extrinsic apoptotic signaling pathway
regulation of neuroinflammatory response
negative regulation of neuroinflammatory response
glial cell-neuron signaling
positive regulation of apoptotic process involved in morphogenesis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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