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Invitrogen
TGF alpha Polyclonal Antibody, Biotin, PeproTech®
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FIGURE: 1 / 1
TGF alpha Antibody (500-P16BT-50UG) in ELISA
Sandwich ELISA: To detect Human TGF-alpha by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of TGF alpha Polyclonal Antibody, Biotin (Product # 500-P16BT-500UG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech TGF alpha Polyclonal Antibody (Product # 500-P16-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human TGF-alpha.
Product Details
500-P16BT-50UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived, 5.5 kDa Recombinant Human TGF-alpha
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: VVSHFNDCPD SHTQFCFHGT CRFLVQEDKP ACVCHSGYVG ARCEHADLLA.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human TGF-alpha. Anti-Human TGF-alpha-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human TGF-alpha by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human TGF-alpha (500-P16) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human TGF-alpha.
Western Blot: To detect Human TGF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human TGF-alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P16BT-1MG will be provided as 2 x 500 µg
Target Information
TGF-alpha is an EGF-related polypeptide growth factor that signals through the EGF receptor, and stimulates the proliferation of a wide range of epidermal and epithelial cells. It is produced by monocytes, keratinocytes, and various tumor cells. TGF-alpha induces anchorage-independence transformation in cultured cells. Human, murine and rat TGF-alpha are cross-species reactive. TGFalpha (aa 50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Protransforming growth factor alpha; TGF a; TGF α; TGF-alpha; TGFalpha; TGFα; Transforming growth factor; transforming growth factor-alpha precursor old gene name 'LU0044C'; unnamed protein product
Gene Aliases: TFGA; TGFA
UniProt ID: (Human) P01135
Entrez Gene ID: (Human) 7039
angiogenesis
cell surface receptor signaling pathway
epidermal growth factor receptor signaling pathway
positive regulation of cell proliferation
intracellular signal transduction
ERBB2-EGFR signaling pathway
positive regulation of MAPK cascade
positive regulation of mitotic nuclear division
positive regulation of epithelial cell proliferation
positive regulation of cell division
mammary gland alveolus development
hepatocyte proliferation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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