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Invitrogen
CXCL16 Polyclonal Antibody, Biotin, PeproTech®
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FIGURE: 1 / 3
CXCL16 Antibody (500-P201GBT-50UG) in WB
Western Blot: To detect Murine CXCL16 by Western Blot analysis CXCL16 Polyclonal Antibody, Biotin (Product # 500-P201GBT-500UG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine CXCL16 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P201GBT-50UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Human
Host/Isotype
Goat
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Murine CXCL16
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: NQGSVAGSCS CDRTISSGTQ IPQGTLDHIR KYLKAFHRCP FFIRFQLQSK SVCGGSQDQW VRELVDCFER KECGTGHGKS FHHQKHLP.
Preparation: Produced from sera of goats immunized with highly pure Recombinant Murine CXCL16. Anti-Murine CXCL16-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect mCXCL16 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine CXCL16 (500-P201G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant mCXCL16.
Western Blot: To detect Murine CXCL16 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine CXCL16 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P201GBT-1MG will be provided as 2 x 500 µg
Target Information
CXCL16 is a member of the CXC chemokine family and signals through the CXCR6 receptor. CXCL16 may play a role in attracting lymphocyte subsets during inflammation and may facilitate certain immune responses. The chemokine domain of CXCL16 contains six cysteine residues, including the four highly conserved cysteine residues characteristic of CXC chemokines. The CXCL16 gene codes for a 273 amino acid polypeptide, which includes a 29 amino acid cytoplasmic domain and transmembrane sequence containing approximately 20 amino acids. The extracellular portion of CXCL16 contains a chemokines domain and an extended C-terminal mucin-like stalk sequence. The extracellular domain contains 89 amino acid residues (86 a.a. residues for the murine homolog).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 16; CC motif chemokine; CCmotif chemokine; CXC; Cxc chemokine ligand 16; CXC motif chemokine; CXCL; Mutant line 498; Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein; Small-inducible cytokine B16; SR-PSOX; SR-PSOX/CXCL16; Transmembrane chemokine CXCL16; zinc finger, MYND-type containing 15
Gene Aliases: 0910001K24Rik; AV290116; b2b498Clo; BB024863; Cxcl16; CXCL16v1; CXCL16v2; SR-PSOX; Srpsox; Zmynd15
UniProt ID: (Mouse) Q8BSU2
Entrez Gene ID: (Mouse) 66102
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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