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Invitrogen
Galectin 1 Polyclonal Antibody, PeproTech®
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FIGURE: 1 / 6
Galectin 1 Antibody (500-P210-1MG) in WB
Western Blot: To detect Human Galectin-1 by Western Blot analysis Galectin 1 Polyclonal Antibody (Product # 500-P210-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Galectin-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P210-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Chicken,
Hamster,
Human,
Mouse
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human Galectin-1
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Amount
1 mg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: ACGLVASNLN LKPGECLRVR GEVAPDAKSF VLNLGKDSNN LCLHFNPRFN AHGDANTIVC NSKDGGAWGT EQREAVFPFQ PGSVAEVCIT FDQANLTVKL PDGYEFKFPN RLNLEAINYM AADGDFKIKC VAFD.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Galectin-1. Anti-Human Galectin-1-specific antibody was purified by affinity chromatography employing an immobilized Human Galectin-1 matrix.
Sandwich ELISA: To detect Human Galectin-1 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human Galectin-1 (500-P210Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Galectin-1.
Western Blot: To detect Human Galectin-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Galectin-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
Galectin-1 belongs to a large family of carbohydrate-binding proteins called lectins. Galectin-1can be either monomeric or homodimeric and is found in a wide variety of cells and tissue types. Galectin-1 can control cell growth, proliferation, induce apoptosis of activated T cells while it can also modulate cytokine secretion or inhibit pro-inflammatory cytokine production. Galectin-1 plays an important role in acute and chronic inflammation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 14 kDa laminin-binding protein; 14 kDa lectin; beta-galactoside binding protein; Beta-galactoside-binding lectin L-14-I; beta-galactoside-binding protein 14kDa; DKFZp686E23103; epididymis secretory sperm binding protein; Gal-1; Galaptin; Galectin-1; Galectin1; H-Galectin-1; HBL; HLBP14; HPL; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; lectin precursor; lectin, galactoside-binding, soluble, 1; LGALS1; pm12; Putative MAPK-activating protein PM12; S-Lac lectin 1; unnamed protein product
Gene Aliases: GAL1; GBP; LGALS1
UniProt ID: (Human) P09382
Entrez Gene ID: (Human) 3956
plasma cell differentiation
apoptotic process
signal transduction
T cell costimulation
regulation of apoptotic process
positive regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
myoblast differentiation
positive regulation of viral entry into host cell
positive regulation of inflammatory response
cell-cell adhesion
negative regulation of T-helper 17 cell lineage commitment
positive regulation of dendritic cell differentiation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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