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Invitrogen

IL-2 Polyclonal Antibody, Biotin, PeproTech®

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IL-2 Polyclonal Antibody, Biotin, PeproTech®

Product Details

500-P22BT-50UG

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.2 µg/mL
-

ELISA (ELISA)

0.25-1.0 µg/mL
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Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human IL-2
3D Epitope / Immunogen

Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Amount

50 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2929926

Product Specific Information

AA Sequence of recombinant protein: MAPTSSSTKK TQLQLEHLLL DLQMILNGIN NYKNPKLTRM LTFKFYMPKK ATELKHLQCL EEELKPLEEV LNLAQSKNFH LRPRDLISNI NVIVLELKGS ETTFMCEYAD ETATIVEFLN RWITFAQSII STLT.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-2. Anti-Human IL-2-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect hIL-2 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IL-2 (500-P22) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hIL-2.

Western Blot: To detect hIL-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hIL-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P22BT-1MG will be provided as 2 x 500 µg

Target Information

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: aldesleukin; cytokine; H-IL-2; il 2; IL-2; ILN; Interleukin; Interleukin-2; Interleukin2; Involved in regulation of T cell clonal expansion; involved in regulation of T-cell clonal expansion; lymphokine; M-IL-2; T cell growth factor; T-cell growth factor; T-cell growth factor (TCGF); TCGF

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Gene Aliases: IL-2; IL2; lymphokine; TCGF

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UniProt ID: (Human) P60568

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Entrez Gene ID: (Human) 3558

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Function(s)
cytokine activity interleukin-2 receptor binding protein binding growth factor activity kinase activator activity carbohydrate binding kappa-type opioid receptor binding glycosphingolipid binding
Process(es)
adaptive immune response leukocyte activation involved in immune response immune system process positive regulation of immunoglobulin production negative regulation of B cell apoptotic process transcription from RNA polymerase II promoter immune response cell adhesion G-protein coupled receptor signaling pathway phospholipase C-activating G-protein coupled receptor signaling pathway positive regulation of cytosolic calcium ion concentration cell-cell signaling positive regulation of cell proliferation gene expression natural killer cell activation T cell differentiation positive regulation of cell growth positive regulation of B cell proliferation positive regulation of interferon-gamma production positive regulation of interleukin-17 production positive regulation of tissue remodeling interleukin-2-mediated signaling pathway T cell proliferation positive regulation of T cell proliferation positive regulation of activated T cell proliferation negative regulation of apoptotic process response to ethanol positive regulation of T cell differentiation positive regulation of regulatory T cell differentiation positive regulation of lymphocyte differentiation positive regulation of transcription from RNA polymerase II promoter regulation of T cell homeostatic proliferation lymphocyte proliferation positive regulation of isotype switching to IgG isotypes negative regulation of lymphocyte proliferation negative regulation of inflammatory response positive regulation of inflammatory response activated T cell proliferation positive regulation of dendritic spine development extrinsic apoptotic signaling pathway in absence of ligand receptor signaling pathway via STAT positive regulation of plasma cell differentiation response to tacrolimus negative regulation of T-helper 17 cell differentiation regulation of CD4-positive, alpha-beta T cell proliferation
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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