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Invitrogen

ApoE3 Polyclonal Antibody, PeproTech®

View all (2) ApoE3 antibodies

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ApoE3 Polyclonal Antibody, PeproTech®

Product Details

500-P238-100UG

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.2 µg/mL
-

ELISA (ELISA)

0.5-2.0 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human ApoE3
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Amount

100 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2929978

Product Specific Information

AA Sequence of recombinant protein: MKVEQAVETE PEPELRQQTE WQSGQRWELA LGRFWDYLRW VQTLSEQVQE ELLSSQVTQE LRALMDETMK ELKAYKSELE EQLTPVAEET RARLSKELQA AQARLGADME DVCGRLVQYR GEVQAMLGQS TEELRVRLAS HLRKLRKRLL RDADDLQKRL AVYQAGAREG AERGLSAIRE RLGPLVEQGR VRAATVGSLA GQPLQERAQA WGERLRARME EMGSRTRDRL DEVKEQVAEV RAKLEEQAQQ IRLQAEAFQA RLKSWFEPLV EDMQRQWAGL VEKVQAAVGT SAAPVPSDNH.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Huma ApoE3. Anti-Human ApoE3-specific antibody was purified by affinity chromatography employing an immobilized Human ApoE3 matrix.

Sandwich ELISA: To detect Human ApoE3 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human ApoE3 (500-P238Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human ApoE3.

Western Blot: To detect Human ApoE3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human ApoE3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

ApoE belongs to a group of proteins that bind reversibly with lipoprotein and play an important role in lipid metabolism. In addition to facilitating solubilization of lipids, these proteins help to maintain the structural integrity of lipoproteins, serve as ligands for lipoprotein receptors, and regulate the activity of enzymes involved in lipid metabolism. Significant quantities of ApoE are produced in the liver and brain, and to some extent in almost every organ. ApoE is an important constituent of all plasma lipoproteins. Its interaction with specific ApoE receptor enables uptake of chylomicron remnants by liver cells, which is an essential step during normal lipid metabolism. It also binds with the LDL receptor (apo B/E). Defects in ApoE are a cause of hyperlipoproteinemia type III. ApoE exists in three major isoforms; E2, E3, and E4, which differ from one another by a single amino-acid substitution. E3 is the most common isoform and is present in 40-90% of the population.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ApoE; apolipoprotein E3; unnamed protein product

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Gene Aliases: AD2; APO-E; ApoE4; LDLCQ5; LPG

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UniProt ID: (Human) P02649

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Entrez Gene ID: (Human) 348

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Function(s)
beta-amyloid binding receptor binding structural molecule activity lipid transporter activity protein binding phospholipid binding heparin binding lipid binding antioxidant activity enzyme binding identical protein binding protein homodimerization activity heparan sulfate proteoglycan binding macromolecular complex binding metal chelating activity protein dimerization activity receptor agonist activity tau protein binding low-density lipoprotein particle receptor binding phosphatidylcholine-sterol O-acyltransferase activator activity very-low-density lipoprotein particle receptor binding lipoprotein particle binding intermembrane cholesterol transfer activity
Process(es)
response to reactive oxygen species retinoid metabolic process negative regulation of endothelial cell proliferation triglyceride metabolic process lipid transport receptor-mediated endocytosis cytoskeleton organization G-protein coupled receptor signaling pathway synaptic transmission, cholinergic long-term memory cholesterol metabolic process negative regulation of platelet activation negative regulation of endothelial cell migration negative regulation of gene expression negative regulation of platelet-derived growth factor receptor signaling pathway positive regulation of cholesterol efflux lipid transport involved in lipid storage positive regulation of neuron projection development negative regulation of neuron projection development long-chain fatty acid transport protein import virion assembly negative regulation of blood coagulation regulation of axon extension neuron projection development melanosome organization regulation of Cdc42 protein signal transduction positive regulation of low-density lipoprotein particle receptor catabolic process cholesterol efflux phospholipid efflux very-low-density lipoprotein particle remodeling low-density lipoprotein particle remodeling high-density lipoprotein particle remodeling high-density lipoprotein particle assembly chylomicron remnant clearance high-density lipoprotein particle clearance very-low-density lipoprotein particle clearance locomotory exploration behavior nitric oxide-cGMP-mediated signaling pathway lipoprotein metabolic process lipoprotein biosynthetic process cholesterol homeostasis regulation of apoptotic process amyloid precursor protein metabolic process regulation of protein complex assembly negative regulation of MAPK cascade negative regulation of blood vessel endothelial cell migration reverse cholesterol transport positive regulation by host of viral process regulation of innate immune response positive regulation of nitric oxide biosynthetic process negative regulation of cholesterol biosynthetic process positive regulation of endocytosis positive regulation of transcription, DNA-templated positive regulation of lipid biosynthetic process regulation of neuronal synaptic plasticity negative regulation of smooth muscle cell proliferation negative regulation of protein secretion negative regulation of inflammatory response regulation of synapse organization positive regulation of membrane protein ectodomain proteolysis negative regulation of lipid biosynthetic process regulation of protein metabolic process negative regulation of protein metabolic process fatty acid homeostasis acylglycerol homeostasis positive regulation of dendritic spine development negative regulation of dendritic spine development regulation of proteasomal protein catabolic process response to caloric restriction triglyceride homeostasis positive regulation of ERK1 and ERK2 cascade cellular response to lipoprotein particle stimulus triglyceride-rich lipoprotein particle clearance intermediate-density lipoprotein particle clearance negative regulation of canonical Wnt signaling pathway regulation of cholesterol metabolic process positive regulation of cholesterol metabolic process negative regulation of cholesterol efflux regulation of plasma lipoprotein particle levels AMPA glutamate receptor clustering NMDA glutamate receptor clustering cellular oxidant detoxification positive regulation of lipoprotein transport regulation of beta-amyloid clearance positive regulation of beta-amyloid clearance negative regulation of long-term synaptic potentiation negative regulation of postsynaptic membrane organization positive regulation of presynaptic membrane organization positive regulation of beta-amyloid formation negative regulation of beta-amyloid formation negative regulation of dendritic spine maintenance positive regulation of dendritic spine maintenance regulation of amyloid precursor protein catabolic process positive regulation of phospholipid efflux positive regulation of neurofibrillary tangle assembly negative regulation of phospholipid efflux negative regulation of lipid transport across blood brain barrier positive regulation of lipid transport across blood brain barrier regulation of cellular response to very-low-density lipoprotein particle stimulus regulation of amyloid fibril formation negative regulation of amyloid fibril formation positive regulation of amyloid fibril formation regulation of behavioral fear response
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