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          • Primary Antibodies ›
          • ApoE3 Antibodies

          Invitrogen

          ApoE3 Polyclonal Antibody, PeproTech®

          View all (2) ApoE3 antibodies

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          Cite ApoE3 Polyclonal Antibody, PeproTech®

          Additional Information:
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          • Antibody Testing Data (3)
          ApoE3 Antibody in Western Blot (WB)
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          ApoE3 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          ApoE3 Antibody (500-P238-50UG) in WB

          Western Blot: To detect Human ApoE3 by Western Blot analysis ApoE3 Polyclonal Antibody (Product # 500-P238-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human ApoE3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          ApoE3 Antibody in Western Blot (WB)
          ApoE3 Antibody in Western Blot (WB)
          ApoE3 Antibody in ELISA (ELISA)
          ApoE3 Polyclonal Antibody, PeproTech®

          Product Details

          500-P238-50UG

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.1-0.2 µg/mL
          -

          ELISA (ELISA)

          0.5-2.0 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Rabbit

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          E.coli-derived Recombinant Human ApoE3
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Lyophilized

          Concentration

          0.1-1.0 mg/mL

          Amount

          50 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS

          Contains

          no preservative

          Storage conditions

          -20°C

          Shipping conditions

          Ambient

          RRID

          AB_2929980

          Product Specific Information

          AA Sequence of recombinant protein: MKVEQAVETE PEPELRQQTE WQSGQRWELA LGRFWDYLRW VQTLSEQVQE ELLSSQVTQE LRALMDETMK ELKAYKSELE EQLTPVAEET RARLSKELQA AQARLGADME DVCGRLVQYR GEVQAMLGQS TEELRVRLAS HLRKLRKRLL RDADDLQKRL AVYQAGAREG AERGLSAIRE RLGPLVEQGR VRAATVGSLA GQPLQERAQA WGERLRARME EMGSRTRDRL DEVKEQVAEV RAKLEEQAQQ IRLQAEAFQA RLKSWFEPLV EDMQRQWAGL VEKVQAAVGT SAAPVPSDNH.

          Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Huma ApoE3. Anti-Human ApoE3-specific antibody was purified by affinity chromatography employing an immobilized Human ApoE3 matrix.

          Sandwich ELISA: To detect Human ApoE3 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human ApoE3 (500-P238Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human ApoE3.

          Western Blot: To detect Human ApoE3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human ApoE3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

          Target Information

          ApoE belongs to a group of proteins that bind reversibly with lipoprotein and play an important role in lipid metabolism. In addition to facilitating solubilization of lipids, these proteins help to maintain the structural integrity of lipoproteins, serve as ligands for lipoprotein receptors, and regulate the activity of enzymes involved in lipid metabolism. Significant quantities of ApoE are produced in the liver and brain, and to some extent in almost every organ. ApoE is an important constituent of all plasma lipoproteins. Its interaction with specific ApoE receptor enables uptake of chylomicron remnants by liver cells, which is an essential step during normal lipid metabolism. It also binds with the LDL receptor (apo B/E). Defects in ApoE are a cause of hyperlipoproteinemia type III. ApoE exists in three major isoforms; E2, E3, and E4, which differ from one another by a single amino-acid substitution. E3 is the most common isoform and is present in 40-90% of the population.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: Apo-E; ApoE; Apolipoprotein E; apolipoprotein E3; unnamed protein product

          View more View less

          Gene Aliases: AD2; APO-E; APOE; ApoE4; LDLCQ5; LPG

          View more View less

          UniProt ID: (Human) P02649

          View more View less

          Entrez Gene ID: (Human) 348

          View more View less

          Function(s)
          beta-amyloid binding receptor binding structural molecule activity lipid transporter activity protein binding phospholipid binding heparin binding lipid binding antioxidant activity enzyme binding identical protein binding protein homodimerization activity heparan sulfate proteoglycan binding macromolecular complex binding metal chelating activity protein dimerization activity receptor agonist activity tau protein binding low-density lipoprotein particle receptor binding phosphatidylcholine-sterol O-acyltransferase activator activity very-low-density lipoprotein particle receptor binding lipoprotein particle binding intermembrane cholesterol transfer activity
          Process(es)
          response to reactive oxygen species negative regulation of endothelial cell proliferation response to dietary excess lipid metabolic process triglyceride metabolic process cholesterol catabolic process lipid transport cellular calcium ion homeostasis receptor-mediated endocytosis response to oxidative stress cytoskeleton organization G-protein coupled receptor signaling pathway synaptic transmission, cholinergic long-term memory steroid metabolic process cholesterol metabolic process gene expression regulation of gene expression negative regulation of platelet activation negative regulation of endothelial cell migration negative regulation of gene expression negative regulation of platelet-derived growth factor receptor signaling pathway positive regulation of cholesterol efflux lipid transport involved in lipid storage positive regulation of neuron projection development negative regulation of neuron projection development long-chain fatty acid transport protein import virion assembly negative regulation of blood coagulation regulation of axon extension neuron projection development melanosome organization regulation of Cdc42 protein signal transduction positive regulation of low-density lipoprotein particle receptor catabolic process cholesterol efflux phospholipid efflux very-low-density lipoprotein particle remodeling low-density lipoprotein particle remodeling high-density lipoprotein particle remodeling high-density lipoprotein particle assembly chylomicron remnant clearance high-density lipoprotein particle clearance very-low-density lipoprotein particle clearance locomotory exploration behavior nitric oxide-cGMP-mediated signaling pathway lipoprotein metabolic process lipoprotein biosynthetic process lipoprotein catabolic process vasodilation cholesterol homeostasis regulation of apoptotic process amyloid precursor protein metabolic process regulation of protein complex assembly negative regulation of MAPK cascade negative regulation of blood vessel endothelial cell migration reverse cholesterol transport positive regulation by host of viral process regulation of innate immune response positive regulation of nitric oxide biosynthetic process negative regulation of cholesterol biosynthetic process positive regulation of endocytosis positive regulation of transcription, DNA-templated positive regulation of lipid biosynthetic process intracellular transport regulation of neuronal synaptic plasticity negative regulation of smooth muscle cell proliferation artery morphogenesis negative regulation of protein secretion negative regulation of inflammatory response regulation of synapse organization positive regulation of membrane protein ectodomain proteolysis negative regulation of lipid biosynthetic process regulation of protein metabolic process negative regulation of protein metabolic process maintenance of location in cell lipid homeostasis fatty acid homeostasis acylglycerol homeostasis artery development positive regulation of dendritic spine development negative regulation of dendritic spine development regulation of proteasomal protein catabolic process response to caloric restriction triglyceride homeostasis positive regulation of ERK1 and ERK2 cascade cellular response to lipoprotein particle stimulus triglyceride-rich lipoprotein particle clearance intermediate-density lipoprotein particle clearance circulatory system development negative regulation of canonical Wnt signaling pathway regulation of cholesterol metabolic process positive regulation of cholesterol metabolic process negative regulation of triglyceride metabolic process negative regulation of cholesterol efflux regulation of plasma lipoprotein particle levels AMPA glutamate receptor clustering NMDA glutamate receptor clustering cellular oxidant detoxification positive regulation of lipoprotein transport regulation of beta-amyloid clearance positive regulation of beta-amyloid clearance negative regulation of long-term synaptic potentiation negative regulation of postsynaptic membrane organization positive regulation of presynaptic membrane organization positive regulation of beta-amyloid formation negative regulation of beta-amyloid formation negative regulation of dendritic spine maintenance positive regulation of dendritic spine maintenance regulation of amyloid precursor protein catabolic process positive regulation of phospholipid efflux positive regulation of neurofibrillary tangle assembly negative regulation of phospholipid efflux negative regulation of lipid transport across blood brain barrier positive regulation of lipid transport across blood brain barrier cellular response to beta-amyloid regulation of cellular response to very-low-density lipoprotein particle stimulus regulation of amyloid fibril formation negative regulation of amyloid fibril formation positive regulation of amyloid fibril formation regulation of behavioral fear response
          It has to be done as per old AB suggested Products section.

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