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Invitrogen

IL-4 Polyclonal Antibody, Biotin, PeproTech®

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IL-4 Polyclonal Antibody, Biotin, PeproTech®

Product Details

500-P24BT-25UG

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.2 µg/mL
-

ELISA (ELISA)

0.25-1.0 µg/mL
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human IL-4
View immunogen

Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Amount

25 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2930057

Product Specific Information

AA Sequence of recombinant protein: MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-4. Anti-Human IL-4-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect hIL-4 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IL-4 (500-P24) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hIL-4.

Western Blot: To detect hIL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hIL-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P24BT-1MG will be provided as 2 x 500 µg

Target Information

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: B cell growth factor 1; B-cell stimulatory factor 1; B_cell stimulatory factor 1; Binetrakin; BSF-1; H-IL-4; IL-4; IL-4v2; IL4; ILN; Interleukin; interleukin 4; Interleukin-4; Interleukin4; Lymphocyte stimulatory factor 1; M-IL-4; MGC79402; Pitrakinra

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Gene Aliases: BCGF-1; BCGF1; BSF-1; BSF1; IL-4; IL4

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UniProt ID: (Human) P05112

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Entrez Gene ID: (Human) 3565

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Function(s)
cytokine activity cytokine receptor binding interleukin-4 receptor binding protein binding growth factor activity
Process(es)
negative regulation of transcription from RNA polymerase II promoter immune response JAK-STAT cascade cholesterol metabolic process positive regulation of cell proliferation positive regulation of gene expression negative regulation of epithelial cell migration positive regulation of macroautophagy B cell differentiation positive regulation of cell migration positive regulation of B cell proliferation negative regulation of tumor necrosis factor production positive regulation of interleukin-10 production positive regulation of interleukin-13 production interleukin-4-mediated signaling pathway type 2 immune response positive regulation of T cell proliferation T cell activation B cell activation macrophage activation myeloid dendritic cell differentiation negative regulation of apoptotic process regulation of isotype switching leukocyte activation positive regulation of MHC class II biosynthetic process positive regulation of T cell differentiation negative regulation of osteoclast differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of receptor-mediated endocytosis positive regulation of isotype switching to IgE isotypes positive regulation of isotype switching to IgG isotypes negative regulation of inflammatory response regulation of immune response regulation of multicellular organismal process regulation of primary metabolic process dendritic cell differentiation positive regulation of cold-induced thermogenesis neuroinflammatory response negative regulation of neuroinflammatory response positive regulation of beta-amyloid clearance positive regulation of cellular respiration negative regulation of complement-dependent cytotoxicity negative regulation of cellular response to transforming growth factor beta stimulus negative regulation of endothelial cell apoptotic process positive regulation of T-helper 2 cell cytokine production positive regulation of ATP biosynthetic process
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