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Invitrogen

WNT3A Polyclonal Antibody, Biotin, PeproTech®

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Product Details

500-P251BT-50UG

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.2 µg/mL
-

ELISA (ELISA)

0.25-1.0 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human Wnt-3a

Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Amount

50 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2930071

Product Specific Information

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Wnt-3a. Anti-Human Wnt-3a-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect hWNT-3a by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human WNT-3a (500-P251) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hWNT-3a.

Western Blot: To detect hWNT-3a by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hWNT-3a is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P251BT-1MG will be provided as 2 x 500 µg

Target Information

Wnt3a, part of the WNT gene family, consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. Study of its expression in the teratocarcinoma cell line NT2 suggests that it may be implicated in the early process of neuronal differentiation of NT2 cells induced by retinoic acid. This gene is clustered with WNT3, another family member, in the chromosome 17q21 region.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: MGC119418; MGC119419; MGC119420; Protein Wnt-3a; unnamed protein product; wingless-type MMTV integration site family, member 3A; Wnt 3a; WNT3A protein

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Gene Aliases: WNT3A

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UniProt ID: (Human) P56704

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Entrez Gene ID: (Human) 89780

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Function(s)
transcription coactivator activity receptor binding frizzled binding cytokine activity protein binding protein domain specific binding co-receptor binding identical protein binding receptor agonist activity
Process(es)
osteoblast differentiation in utero embryonic development somitogenesis positive regulation of cytokine production heart looping positive regulation of receptor internalization transcription from RNA polymerase II promoter determination of left/right symmetry axonogenesis axon guidance mesoderm development heart development protein localization positive regulation of cell proliferation anterior/posterior pattern specification COP9 signalosome assembly regulation of gene expression positive regulation of gene expression negative regulation of neuron projection development Wnt signaling pathway spinal cord association neuron differentiation hippocampus development cell proliferation in forebrain Wnt signaling pathway involved in forebrain neuroblast division dorsal/ventral neural tube patterning neurogenesis hemopoiesis platelet activation neuron differentiation extracellular matrix organization mammary gland development positive regulation of B cell proliferation midbrain development cell proliferation in midbrain non-canonical Wnt signaling pathway skeletal muscle cell differentiation post-anal tail morphogenesis synaptic vesicle recycling B cell proliferation inner ear morphogenesis cell fate commitment fat cell differentiation myoblast differentiation regulation of cell differentiation negative regulation of fat cell differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter somatic stem cell division positive regulation of mesodermal cell fate specification paraxial mesodermal cell fate commitment animal organ development positive regulation of skeletal muscle tissue development positive regulation of collateral sprouting in absence of injury negative regulation of axon extension involved in axon guidance negative regulation of neurogenesis regulation of axonogenesis modulation of synaptic transmission regulation of synapse organization canonical Wnt signaling pathway cardiac muscle cell fate commitment positive regulation of dermatome development secondary palate development regulation of microtubule cytoskeleton organization platelet aggregation cellular response to retinoic acid axis elongation involved in somitogenesis positive regulation of canonical Wnt signaling pathway calcium ion transmembrane transport via low voltage-gated calcium channel presynapse assembly positive regulation of protein localization to plasma membrane negative regulation of dopaminergic neuron differentiation midbrain dopaminergic neuron differentiation regulation of postsynapse to nucleus signaling pathway regulation of presynapse assembly positive regulation of cell-cell adhesion mediated by cadherin positive regulation of neural precursor cell proliferation positive regulation of hepatocyte proliferation positive regulation of cardiac muscle cell differentiation regulation of RNA biosynthetic process
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