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Invitrogen
beta-Defensin 1 Polyclonal Antibody, Biotin, PeproTech®
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FIGURE: 1 / 3
beta-Defensin 1 Antibody (500-P253BT-500UG) in WB
Western Blot: To detect hBD-1 by Western Blot analysis beta-Defensin 1 Polyclonal Antibody, Biotin (Product # 500-P253BT-500UG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hBD-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P253BT-500UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human BD-1 (47 a.a.)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Amount
500 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: GNFLTGLGHR SDHYNCVSSG GQCLYSACPI FTKIQGTCYR GKAKCCK.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human BD-1. Anti-Human BD-1-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hBD-1 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human BD-1 (500-P253) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hBD-1.
Western Blot: To detect hBD-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hBD-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P253BT-1MG will be provided as 2 x 500 µg
Target Information
Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 1, an antimicrobial peptide implicated in the resistance of epithelial surfaces to microbial colonization. This gene maps in close proximity to defensin family member, defensin, alpha 1 and has been implicated in the pathogenesis of cystic fibrosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BD-1; Beta-defensin 1; Defensin, beta 1; hBD-1
Gene Aliases: BD1; DEFB-1; DEFB1; DEFB101; HBD1
UniProt ID: (Human) P60022
Entrez Gene ID: (Human) 1672
innate immune response in mucosa
chemotaxis
defense response
immune response
G-protein coupled receptor signaling pathway
response to bacterium
calcium-mediated signaling
antibacterial humoral response
defense response to bacterium
innate immune response
defense response to Gram-negative bacterium
defense response to Gram-positive bacterium
positive regulation of sperm motility involved in capacitation
antimicrobial humoral immune response mediated by antimicrobial peptide
defense response to symbiont
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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