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Invitrogen

p16INK4a-TAT Polyclonal Antibody, PeproTech®

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p16INK4a-TAT Polyclonal Antibody, PeproTech®

Product Details

500-P284T-100UG

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.2 µg/mL
-

ELISA (ELISA)

0.5-2.0 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human p16-INK4a-TAT
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Amount

100 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2930235

Product Specific Information

AA Sequence of recombinant protein: EPAAGSSMEP SADWLATAAA RGRVEEVRAL LEAGALPNAP NSYGRRPIQV MMMGSARVAE LLLLHGAEPN CADPATLTRP VHDAAREGFL DTLVVLHRAG ARLDVRDAWG RLPVDLAEEL GHRDVARYLR AAAGGTRGSN HARIDAAEGP SDIPDGYGR KKRRQRRR.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human p16-INK4a-TAT. Anti-Human p16-INK4a-TAT-specific antibody was purified by affinity chromatography employing an immobilized Human p16-INK4a-TAT matrix.

Sandwich ELISA: To detect Human p16-INK4a-TAT by sandwich ELISA (using100 µL/well antibody solution), a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human p16-INK4a-TAT (500-P284TBt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human p16-INK4a-TAT.

Western Blot: To detect Human p16-INK4a-TAT by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL.When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human p16-INK4a-TAT is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

p16-INK4a is a nuclear protein that regulates the cell cycle by inhibiting cyclin-dependent kinase-4 (CDK4) and CDK6. p16-INK4a inhibits CDK activity by binding to the CDK molecules in a manner that interferes with their ability to interact with cyclin D. This activity has the effect of suppressing tumor formation and growth, and of inducing replicative senescence in various normal cells, including stem cells. The expression of p16-INK4a steadily increases with age, and tends to accumulate in stem cell compartments. The deletion, rearrangement, or mutation of the p16-INK4a gene is frequently found in melanomas, as well as in certain other types of cancer. p16-INK4a and other transcription factors have been introduced into cells by DNA transfection, viral infection, or microinjection. Protein transduction using TAT fusion proteins represents an alternative methodology for introducing transcription factors and other nuclear proteins into primary, as well as transformed, cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: a frameshift between exon 1 (0.18) and exon 2 changed the ORF of p16INK4 gene.; Alternative reading frame; ARF; CDK4 inhibitor p16-INK4; CDK4I; CDKN2A/ARF Intron 2 lncRNA; cell cycle negative regulator beta; Cyclin-dependent kinase 4 inhibitor A; Cyclin-dependent kinase inhibitor 2A; cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4); hypothetical 18.1 kDa protein from longest open reading frame; inhibitor of cdk4 A; MTS-1; Multiple tumor suppressor 1; multiple tumour suppressor 1; p14ARF; p16; p16 gamma; p16-INK4; p16-INK4a; p16INK4a; Tumor suppressor ARF

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Gene Aliases: ARF; CAI2; CDK4I; CDKN2; CDKN2A; CMM2; INK4; INK4A; MLM; MTS-1; MTS1; P14; P14ARF; P16; P16-INK4A; P16INK4; P16INK4A; P19; P19ARF; TP16

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UniProt ID: (Human) P42771

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Entrez Gene ID: (Human) 1029

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Function(s)
p53 binding DNA binding RNA binding cyclin-dependent protein serine/threonine kinase inhibitor activity protein binding SUMO transferase activity protein kinase binding NF-kappaB binding ligase inhibitor activity ubiquitin-protein transferase inhibitor activity RNA polymerase II sequence-specific DNA binding transcription factor binding MDM2/MDM4 family protein binding disordered domain specific binding ubiquitin ligase inhibitor activity
Process(es)
negative regulation of transcription from RNA polymerase II promoter protein polyubiquitination mitophagy negative regulation of cell-matrix adhesion rRNA processing apoptotic process Ras protein signal transduction negative regulation of cell proliferation epidermis development rRNA transcription regulation of gene expression positive regulation of gene expression protein sumoylation keratinocyte differentiation negative regulation of cell growth nuclear body organization negative regulation of B cell proliferation regulation of protein stability protein destabilization negative regulation of immature T cell proliferation in thymus positive regulation of protein sumoylation mammary gland epithelial cell proliferation negative regulation of mammary gland epithelial cell proliferation positive regulation of smooth muscle cell apoptotic process protein localization to nucleus somatic stem cell population maintenance glucose homeostasis positive regulation of apoptotic process positive regulation of DNA damage response, signal transduction by p53 class mediator keratinocyte proliferation negative regulation of cyclin-dependent protein serine/threonine kinase activity negative regulation of cell cycle negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter regulation of nucleocytoplasmic transport regulation of protein export from nucleus somatic stem cell division protein stabilization regulation of cell cycle mitochondrial depolarization apoptotic process involved in mammary gland involution positive regulation of apoptotic process involved in mammary gland involution cellular response to hydrogen peroxide protein K63-linked ubiquitination cellular senescence replicative senescence oncogene-induced cell senescence apoptotic signaling pathway positive regulation of protein localization to nucleus positive regulation of signal transduction by p53 class mediator protein localization to nucleolus negative regulation of proteolysis involved in cellular protein catabolic process regulation of protein targeting to mitochondrion negative regulation of ubiquitin protein ligase activity amyloid fibril formation regulation of G1/S transition of mitotic cell cycle negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process positive regulation of macrophage apoptotic process negative regulation of protein neddylation
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