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Invitrogen
IL-9 Polyclonal Antibody, Biotin, PeproTech®
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FIGURE: 1 / 3
IL-9 Antibody (500-P29BT-50UG) in WB
Western Blot: To detect Human IL-9 by Western Blot analysis IL-9 Polyclonal Antibody, Biotin (Product # 500-P29BT-500UG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IL-9 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P29BT-50UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human IL-9
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: MQGCPTLAGI LDINFLINKM QEDPASKCHC SANVTSCLCL GIPSDNCTRP CFSERLSQMT NTTMQTRYPL IFSRVKKSVE VLKNNKCPYF SCEQPCNQTT AGNALTFLKS LLEIFQKEKM RGMRGKI.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-9. Anti-Human IL-9-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human IL-9 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IL-9 (500-P29) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-9.
Western Blot: To detect Human IL-9 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IL-9 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P29BT-1MG will be provided as 2 x 500 µg
Target Information
Interleukin 9 (IL-9) is produced by activated T helper 2 (Th2) lymphocytes. IL-9 regulates many cell types involved in classical Th2-associated asthma responses, including B and T lymphocytes, mast cells, eosinophils, and epithelial cells. The mouse IL-9 cDNA encodes a 144 amino acid precursor protein with an 18 amino acid signal peptide that is cleaved to form the mature cysteine-rich protein with a molecular mass of 14 kDa. Native mouse IL-9 is heavily glycosylated. Recently, TGF beta has been reported to play an important role in the differentiation of IL-17-producing T helper cells and inducible regulatory T cells by reprogramming Th2 cells to secrete IL-9, thereby driving the differentiation of Th9 cells directly.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cytokine P40; homolog of mouse T cell and mast cell growth factor 40; homolog of mouse T cell and mast cell growth factor P40; IL-9; ILN; Interleukin; Interleukin-9; Interleukin9; p40 cytokine; P40 protein precursor; p40 T-cell and mast cell growth factor; T-cell growth factor P40
Gene Aliases: HP40; IL-9; IL9; P40
UniProt ID: (Human) P15248
Entrez Gene ID: (Human) 3578
inflammatory response
immune response
positive regulation of cell proliferation
immunoglobulin mediated immune response
B cell differentiation
positive regulation of cell growth
positive regulation of interleukin-5 production
interleukin-9-mediated signaling pathway
B cell proliferation
regulation of JAK-STAT cascade
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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