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Invitrogen

EGFR Polyclonal Antibody, Biotin, PeproTech®

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EGFR Polyclonal Antibody, Biotin, PeproTech®

Product Details

500-P306BT-25UG

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.2 µg/mL
-

ELISA (ELISA)

0.25-1.0 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

CHO cells-derived Recombinant Human EGF Receptor (EGFR)
View immunogen

Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Amount

25 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2930318

Product Specific Information

AA Sequence of recombinant protein: LEEKKVCQGT SNKLTQLGTF EDHFLSLQRM FNNCEVVLGN LEITYVQRNY DLSFLKTIQE VAGYVLIALN TVERIPLENL QIIRGNMYYE NSYALAVLSN YDANKTGLKE LPMRNLQEIL HGAVRFSNNP ALCNVESIQW RDIVSSDFLS NMSMDFQNHL GSCQKCDPSC PNGSCWGAGE ENCQKLTKII CAQQCSGRCR GKSPSDCCHN QCAAGCTGPR ESDCLVCRKF RDEATCKDTC PPLMLYNPTT YQMDVNPEGK YSFGATCVKK CPRNYVVTDH GSCVRACGAD SYEMEEDGVR KCKKCEGPCR KVCNGIGIGE FKDSLSINAT NIKHFKNCTS ISGDLHILPV AFRGDSFTHT PPLDPQELDI LKTVKEITGF LLIQAWPENR TDLHAFENLE IIRGRTKQHG QFSLAVVSLN ITSLGLRSLK EISDGDVIIS GNKNLCYANT INWKKLFGTS GQKTKIISNR GENSCKATGQ VCHALCSPEG CWGPEPRDCV SCRNVSRGRE CVDKCNLLEG EPREFVENSE CIQCHPECLP QAMNITCTGR GPDNCIQCAH YIDGPHCVKT CPAGVMGENN TLVWKYADAG HVCHLCHPNC TYGCTGPGLE GCPTNGPKIP S.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human EGF Receptor (EGFR). Anti-Human EGF Receptor (EGFR)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect Human EGF Receptor by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human EGF Receptor (500-P306) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human EGF Receptor.

Western Blot: To detect Human EGF Receptor by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human EGF Receptor is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P306BT-1MG will be provided as 2 x 500 µg

Target Information

EGFR, epidermal growth factor receptor, is a receptor tyrosine kinases that signals in response to various growth factors. Overexpression has been linked to numerous types of cancer and EGFR is the target of both biological and small molecular therapeutics. EGFR is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 2.7.10.1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; contains extracellular domain; EC 2.7.10.1; EGFR vIII; Epidermal growth factor receptor; epidermal growth factor receptor tyrosine kinase domain; erb-b2 receptor tyrosine kinase 1; GIG40; kinase EGFR; membrane protein; Oncogene ERBB; PIG61; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; unnamed protein product; Urogastrone

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Gene Aliases: EGFR; ERBB; ERBB1; ERRP; HER1; mENA; NISBD2; NNCIS; PIG61

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UniProt ID: (Human) P00533

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Entrez Gene ID: (Human) 1956

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Function(s)
nucleotide binding virus receptor activity chromatin binding double-stranded DNA binding protein kinase activity MAP kinase kinase kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity transmembrane signaling receptor activity epidermal growth factor-activated receptor activity protein binding ATP binding kinase activity transferase activity enzyme binding kinase binding protein phosphatase binding protein tyrosine kinase activator activity ubiquitin protein ligase binding identical protein binding cadherin binding epidermal growth factor binding actin filament binding ATPase binding
Process(es)
MAPK cascade cell morphogenesis ossification embryonic placenta development positive regulation of protein phosphorylation hair follicle development signal transduction cell surface receptor signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway epidermal growth factor receptor signaling pathway salivary gland morphogenesis learning or memory cell proliferation positive regulation of cell proliferation epidermis development cerebral cortex cell migration neuron differentiation positive regulation of cell growth positive regulation of cell migration positive regulation of peptidyl-serine phosphorylation ERBB2-EGFR signaling pathway negative regulation of epidermal growth factor receptor signaling pathway regulation of cell proliferation negative regulation of protein catabolic process positive regulation of phosphorylation negative regulation of apoptotic process positive regulation of MAPK cascade protein kinase B signaling positive regulation of DNA repair positive regulation of DNA replication positive regulation of transcription from RNA polymerase II promoter viral entry into host cell positive regulation of fibroblast proliferation digestive tract morphogenesis epithelial cell proliferation positive regulation of epithelial cell proliferation regulation of peptidyl-tyrosine phosphorylation protein insertion into membrane positive regulation of protein kinase B signaling morphogenesis of an epithelial fold eyelid development in camera-type eye response to UV-A positive regulation of ERK1 and ERK2 cascade cellular response to amino acid stimulus cellular response to growth factor stimulus cellular response to epidermal growth factor stimulus cellular response to estradiol stimulus positive regulation of protein kinase C signaling positive regulation of canonical Wnt signaling pathway cell-cell adhesion positive regulation of G1/S transition of mitotic cell cycle positive regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of protein localization to plasma membrane negative regulation of cardiocyte differentiation
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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