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Invitrogen
Osteopontin Polyclonal Antibody, PeproTech®
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FIGURE: 1 / 3
Osteopontin Antibody (500-P314-50UG) in WB
Western Blot: To detect Human Osteopontin by Western Blot analysis Osteopontin Polyclonal Antibody (Product # 500-P314-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human Osteopontin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P314-50UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
HEK293 cells-derived Recombinant Human Osteopontin
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: IPVKQADSGS SEEKQLYNKY PDAVATWLNP DPSQKQNLLA PQNAVSSEET NDFKQETLPS KSNESHDHMD DMDDEDDDDH VDSQDSIDSN DSDDVDDTDD SHQSDESHHS DESDELVTDF PTDLPATEVF TPVVPTVDTY DGRGDSVVYG LRSKSKKFRR PDIQYPDATD EDITSHMESE ELNGAYKAIP VAQDLNAPSD WDSRGKDSYE TSQLDDQSAE THSHKQSRLY KRKANDESNE HSDVIDSQEL SKVSREFHSH EFHSHEDMLV VDPKSKEEDK HLKFRISHEL DSASSEVN.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Osteopontin. Anti-Human Osteopontin-specific antibody was purified by affinity chromatography employing an immobilized Human Osteopontin matrix.
Sandwich ELISA: To detect Human Osteopontin by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human Osteopontin (500-P314Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Osteopontin.
Western Blot: To detect Human Osteopontin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human Osteopontin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
Osteopontin is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein that interacts with integrins and CD44 as major receptors. It is a multifunctional protein involved in bone mineralization, cell adhesion, cell migration, chronic inflammatory disease and transformation. Proteolytic cleavage by thrombin and matrix metalloproteinases close to the integrin-binding Arg-Gly-Asp sequence modulates the function of OPN and its integrin binding properties. Thrombin-cleaved fragments of Osteopontin are overexpressed in malignant glial tumors and provide a molecular niche with survival advantage and provide a novel substrate for plasmin and cathepsin D.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Bone sialoprotein 1; Bone sialoprotein 1 (BSPI/BSP1); CALPHA1 fusion; Early T lymphocyte activation 1 (ETA1); early T-lymphocyte activation 1; HGNC:11255; immunoglobulin alpha 1 heavy chain constant region fusion protein; lnc-PKD2-2-3; MGC110940; Nephropontin; OSN-C; OSN-D; Osteopontin; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; putative OPN isoform 6; Secreted phosphoprotein 1; secreted phosphoprotein 1 (osteopontin bone sialoprotein I early T lymphocyte activation 1); secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); Secreted phosphoprotein 1 (SPP1); SPP-1; SPP1/CALPHA1 fusion; unnamed protein product; urinar; Urinary stone protein; Uropontin
Gene Aliases: BNSP; BSPI; ETA-1; OPN; PSEC0156; SPP1
UniProt ID: (Human) P10451
Entrez Gene ID: (Human) 6696
ossification
osteoblast differentiation
androgen catabolic process
cell adhesion
signal transduction
embryo implantation
cell differentiation
biomineral tissue development
response to vitamin D
response to macrophage colony-stimulating factor
positive regulation of bone resorption
positive regulation of transcription, DNA-templated
decidualization
response to steroid hormone
negative regulation of collateral sprouting of intact axon in response to injury
cellular response to testosterone stimulus
response to 2,3,7,8-tetrachlorodibenzodioxine
positive regulation of estradiol secretion
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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