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Invitrogen
CD14 (soluble) Polyclonal Antibody, Biotin, PeproTech®
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FIGURE: 1 / 3
CD14 (soluble) Antibody (500-P320BT-50UG) in WB
Western Blot: To detect Human sCD14 by Western Blot analysis CD14 (soluble) Polyclonal Antibody, Biotin (Product # 500-P320BT-500UG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human sCD14 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P320BT-50UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
HEK293 cells-derived Recombinant Human sCD14
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: TTPEPCELDD EDFRCVCNFS EPQPDWSEAF QCVSAVEVEI HAGGLNLEPF LKRVDADADP RQYADTVKAL RVRRLTVGAA QVPAQLLVGA LRVLAYSRLK ELTLEDLKIT GTMPPLPLEA TGLALSSLRL RNVSWATGRS WLAELQQWLK PGLKVLSIAQ AHSPAFSCEQ VRAFPALTSL DLSDNPGLGE RGLMAALCPH KFPAIQNLAL RNTGMETPTG VCAALAAAGV QPHSLDLSHN SLRATVNPSA PRCMWSSALN SLNLSFAGLE QVPKGLPAKL RVLDLSCNRL NRAPQPDELP EVDNLTLDGN PFLVPGTALP HEGSMNSGVV P.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human sCD14. Anti-Human CD14-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human sCD14 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human sCD14 (500-P320) as a capture antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human sCD14.
Western Blot: To detect Human sCD14 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human sCD14 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P320BT-1MG will be provided as 2 x 500 µg
Target Information
CD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils. CD14 also serves as a multifunctional lipopolysaccharide receptor, and is released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 can discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. Further, CD14 has been shown to bind apoptotic cells, and can affect allergic, inflammatory and infectious processes. Alternative splicing results in multiple transcript variants encoding the same CD14 isoform. Diseases associated with CD14 dysfunction include mycobacterium chelonae infection and Croup.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD 14; CD14; cd14 monocyte; LPSR antibody; Monocyte differentiation antigen CD14; My23 antigen; Myeloid cell-specific leucine-rich glycoprotein; sCD14; soluble CD14; unnamed protein product
Gene Aliases: CD14
UniProt ID: (Human) P08571
Entrez Gene ID: (Human) 929
positive regulation of cytokine production
response to molecule of bacterial origin
immune system process
cell surface pattern recognition receptor signaling pathway
receptor-mediated endocytosis
phagocytosis
apoptotic process
inflammatory response
cell surface receptor signaling pathway
response to bacterium
lipopolysaccharide-mediated signaling pathway
positive regulation of lipopolysaccharide-mediated signaling pathway
positive regulation of type I interferon production
positive regulation of interferon-gamma production
positive regulation of interleukin-8 production
positive regulation of tumor necrosis factor production
toll-like receptor 4 signaling pathway
positive regulation of toll-like receptor 4 signaling pathway
innate immune response
positive regulation of endocytosis
cellular response to molecule of bacterial origin
cellular response to lipopolysaccharide
cellular response to lipoteichoic acid
cellular response to diacyl bacterial lipopeptide
cellular response to triacyl bacterial lipopeptide
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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