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Invitrogen
MMP3 Polyclonal Antibody, PeproTech®
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FIGURE: 1 / 3
MMP3 Antibody (500-P324-100UG) in WB
Western Blot: To detect Human MMP-3 by Western Blot analysis MMP3 Polyclonal Antibody (Product # 500-P324-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human MMP-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P324-100UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human MMP-3
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: MRTFPGIPKW RKTHLTYRIV NYTPDLPKDA VDSAVEKALK VWEEVTPLTF SRLYEGEADI MISFAVREHG DFYPFDGPGN VLAHAYAPGP GINGDAHFDD DEQWTKDTTG TNLFLVAAHE IGHSLGLFHS ANTEALMYPL YHSLTDLTRF RLSQDDINGI QSLYGPPPDS PETPLVPTEP VPPEPGTPAN CDPALSFDAV STLRGEILIF KDRHFWRKSL RKLEPELHLI SSFWPSLPSG VDAAYEVTSK DLVFIFKGNQ FWAIRGNEVR AGYPRGIHTL GFPPTVRKID AAISDKEKNK TYFFVEDKYW RFDEKRNSME PGFPKQIAED FPGIDSKIDA VFEEFGFFYF FTGSSQLEFD PNAKKVTHTL KSNSWLNC.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human MMP-3. Anti-Human MMP-3-specific antibody was purified by affinity chromatography employing an immobilized Human MMP-3 matrix.
Sandwich ELISA: To detect Human MMP-3 by sandwich ELISA (using 100 µL/well), a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human MMP-3 (500-P324BT) as a detection antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human MMP-3.
Western Blot: To detect Human MMP-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human MMP-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
MMP3 (stromelysin-1) belongs to the peptidase M10A subfamily. It is secreted in its pro-protein form, and is activated by extracellular proteinase cleavage. MMP3 is capable of binding to zinc and calcium ions, and has metallo-endopeptidase activity. The active enzyme degrades collagen, gelatin, fibronectin, and laminin. It is involved in normal cellular processes such as wound repair and tissue remodeling. Mutations in the gene can result in coronary heart disease 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EC 3.4.24.17; matrix metallo protease; matrix metalloproteinase 3 (stromelysin 1, progelatinase); Matrix metalloproteinase-3; MMP; MMP-3; MMPs; proteoglycanase; SL-1; Stromelysin-1; Stromelysin1; Transin-1; Transin1; unnamed protein product
Gene Aliases: CHDS6; MMP-3; MMP3; SL-1; STMY; STMY1; STR1
UniProt ID: (Human) P08254
Entrez Gene ID: (Human) 4314
immune system process
proteolysis
extracellular matrix disassembly
protein catabolic process
extracellular matrix organization
regulation of cell migration
collagen catabolic process
positive regulation of protein complex assembly
cellular response to reactive oxygen species
innate immune response
negative regulation of protein kinase B signaling
cellular response to lipopolysaccharide
cellular response to amino acid stimulus
cellular response to UV-A
cellular response to nitric oxide
regulation of neuroinflammatory response
response to amyloid-beta
negative regulation of reactive oxygen species metabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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