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Invitrogen
GM-CSF Polyclonal Antibody, Biotin, PeproTech®
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FIGURE: 1 / 3
GM-CSF Antibody (500-P33BT-25UG) in WB
Western Blot: To detect hGM-CSF by Western Blot analysis GM-CSF Polyclonal Antibody, Biotin (Product # 500-P33BT-500UG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hGM-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P33BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human GM-CSF
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Amount
25 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNET VEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTM MASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPF DCWEPVQE.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human GM-CSF. Anti-Human GM-CSF-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hGM-CSF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human GM-CSF (500-P33) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hGM-CSF.
Western Blot: To detect hGM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hGM-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P33BT-1MG will be provided as 2 x 500 µg
Target Information
The GM-CSF gene encodes for a cytokine that regulates the production, differentiation, and function of macrophages and granulocytes. The active form of the protein exists as a homodimer in the extracellular space. This gene is located in a cluster of related genes at the chromosome region 5q31, which has been associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include interleukins 4, 5, and 13. The gene is involved in promoting tissue inflammation. Elevated levels of cytokines, including the one produced by this gene, have been observed in SARS-CoV-2 infected patients with acute respiratory distress syndrome. Mice that lack this gene or its receptor have been shown to develop pulmonary alveolar proteinosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: colony stimulating factor 2 (granulocyte-macrophage); Colony-stimulating factor; CSF; GM-CSF; granulocyte macrophage colony stimulating factor; granulocyte macrophage-colony stimulating factor; Granulocyte-macrophage colony-stimulating factor; granulocyte/macrophage colony-stimulating factor; M-GM-CSF; MGC131935; MGC138897; MGC151255; MGC151257; molgramostim; Molgramostin; Sargramostim
Gene Aliases: CSF; CSF2; GMCSF
UniProt ID: (Human) P04141
Entrez Gene ID: (Human) 1437
histamine secretion
embryonic placenta development
immune response
signal transduction
JAK-STAT cascade
cell proliferation
positive regulation of cell proliferation
regulation of signal transduction
regulation of gene expression
positive regulation of gene expression
positive regulation of macrophage derived foam cell differentiation
myeloid cell differentiation
neutrophil differentiation
monocyte differentiation
macrophage differentiation
positive regulation of cell migration
positive regulation of interleukin-23 production
response to silicon dioxide
response to fluid shear stress
granulocyte-macrophage colony-stimulating factor signaling pathway
epithelial fluid transport
regulation of cell proliferation
myeloid dendritic cell differentiation
regulation of circadian sleep/wake cycle, sleep
negative regulation of transcription, DNA-templated
regulation of developmental process
positive regulation of leukocyte proliferation
cellular response to lipopolysaccharide
positive regulation of podosome assembly
cellular response to granulocyte macrophage colony-stimulating factor stimulus
dendritic cell differentiation
receptor signaling pathway via STAT
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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