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Invitrogen
MCP-3 Polyclonal Antibody, Biotin, PeproTech®
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FIGURE: 1 / 3
MCP-3 Antibody (500-P37GBT-50UG) in WB
Western Blot: To detect by Western Blot analysis MCP-3 Polyclonal Antibody, Biotin (Product # 500-P37GBT-500UG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MCP-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
500-P37GBT-50UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Goat
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human MCP-3 (CCL7)
Conjugate
Biotin
Biotin
Biotin
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: QPVGINTSTT CCYRFINKKI PKQRLESYRR TTSSHCPREA VIFKTKLDKE ICADPTQKWV QDFMKHLDKK TQTPKL.
Preparation: Produced from sera of goats immunized with highly pure Recombinant Human MCP-3 (CCL7). Anti-Human MCP-3 (CCL7)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hMCP-3 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human MCP-3 (500-P37G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hMCP-3.
Western Blot: To detect by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MCP-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P37GBT-1MG will be provided as 2 x 500 µg
Target Information
This is a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: chemokine (C-C motif) ligand 7; H-MCP-3; MGC138463; MGC138465; monocyte chemoattractant protein 3; monocyte chemotactic protein 3; RP23-350G1.4; small inducible cytokine A7 (monocyte chemotactic protein 3); small-inducible cytokine A7
Gene Aliases: FIC; MARC; MCP-3; MCP3; NC28; SCYA6; SCYA7
UniProt ID: (Human) P80098
Entrez Gene ID: (Human) 6354
monocyte chemotaxis
cellular calcium ion homeostasis
chemotaxis
inflammatory response
immune response
cytoskeleton organization
signal transduction
cell-cell signaling
regulation of cell shape
response to gamma radiation
positive regulation of cell migration
eosinophil chemotaxis
positive regulation of inflammatory response
antimicrobial humoral immune response mediated by antimicrobial peptide
chemokine-mediated signaling pathway
cellular response to ethanol
positive regulation of natural killer cell chemotaxis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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