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Invitrogen
Eotaxin Polyclonal Antibody, Biotin, PeproTech®
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FIGURE: 1 / 3
Eotaxin Antibody (500-P41GBT-25UG) in WB
Western Blot: To detect hEotaxin by Western Blot analysis Eotaxin Polyclonal Antibody, Biotin (Product # 500-P41GBT-500UG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hEotaxin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P41GBT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human Eotaxin (CCL11)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Amount
25 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: GPASVPTTCC FNLANRKIPL QRLESYRRIT SGKCPQKAVI FKTKLAKDIC ADPKKKWVQD SMKYLDQKSP TPKP.
Preparation: Produced from sera of goats immunized with highly pure Recombinant Human Eotaxin (CCL11). Anti-Human Eotaxin (CCL11)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hEotaxin by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Eotaxin (500-P41G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hEotaxin.
Western Blot: To detect hEotaxin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hEotaxin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P41GBT-1MG will be provided as 2 x 500 µg
Target Information
This gene is one of several Cys-Cys (CC) cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for eosinophils, but not mononuclear cells or neutrophils. This eosinophil specific chemokine assumed to be involved in eosinophilic inflammatory diseases such as atopic dermatitis, allergic rhinitis, asthma and parasitic infections.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-C motif chemokine 11; CCL11; chemokine (C-C motif) ligand 11; Eosinophil Chemotactic Factor; Eosinophil chemotactic protein; Eotaxin; Eotaxin 1; eotaxin-1; Eotaxin1; MGC22554; small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin); Small-inducible cytokine A11
Gene Aliases: CCL11; SCYA11
UniProt ID: (Human) P51671
Entrez Gene ID: (Human) 6356
positive regulation of endothelial cell proliferation
chronic inflammatory response
mast cell chemotaxis
protein phosphorylation
cellular calcium ion homeostasis
chemotaxis
inflammatory response
immune response
cytoskeleton organization
actin filament organization
cell adhesion
signal transduction
learning or memory
regulation of cell shape
response to radiation
response to virus
positive regulation of cell migration
positive regulation of actin filament polymerization
killing of cells of other organism
response to interleukin-13
positive regulation of angiogenesis
eosinophil chemotaxis
negative regulation of neurogenesis
branching involved in mammary gland duct morphogenesis
mammary duct terminal end bud growth
antimicrobial humoral immune response mediated by antimicrobial peptide
chemokine-mediated signaling pathway
ERK1 and ERK2 cascade
response to interleukin-4
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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