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Invitrogen
EGF Polyclonal Antibody, PeproTech®
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FIGURE: 1 / 3
EGF Antibody (500-P45-100UG) in WB
Western Blot: To detect Human EGF by Western Blot analysis EGF Polyclonal Antibody (Product # 500-P45-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human EGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P45-100UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human EGF
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human EGF. Anti-Human EGF-specific antibody was purified by affinity chromatography employing an immobilized Human EGF matrix.
Sandwich ELISA: To detect Human EGF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human EGF (500-P45Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human EGF.
Western Blot: To detect Human EGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human EGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
EGF (Epidermal growth factor) exerts its actions by binding to the EGF Receptor, a 170 kDa protein kinase. Activation of EGFR initiates diverse cellular pathways in response to toxic environmental stimuli, or to EGF binding to the receptor, the EGFR forms homo- or heterodimers with other family members. Each dimeric receptor complex initiates a distinct signaling pathway by recruiting different Src homology 2 (SH2) containing effector proteins. EGF is far and wide expressed in kidney, cerebrum, prostrate and salivary glands. EGF acts as a potent mitogenic factor and the phosphorylated receptor recruits adapter proteins like GRB2 that activates complex downstream signaling cascades. EGF activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STAT modules. Research studies suggest the protein may also play important role in activating the NF-kappa-B signaling cascade. Defects in the EGGF gene are the cause of hypomagnesemia type 4 and dysregulation has been associated with the growth and progression of certain cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: beta-urogastrone; EGF; Epidermal growth factor; H-EGF; Pro-epidermal growth factor; unnamed protein product
Gene Aliases: EGF; HOMG4; URG
UniProt ID: (Human) P01133
Entrez Gene ID: (Human) 1950
angiogenesis
positive regulation of endothelial cell proliferation
positive regulation of receptor internalization
epidermal growth factor receptor signaling pathway
cell proliferation
positive regulation of cell proliferation
positive regulation of endothelial cell migration
positive regulation of macromolecule metabolic process
positive regulation of gene expression
positive regulation of peptidyl-threonine phosphorylation
cerebellar granule cell precursor proliferation
positive regulation of cerebellar granule cell precursor proliferation
positive regulation of cell migration
ERBB2-EGFR signaling pathway
positive regulation of phosphorylation
positive regulation of DNA binding
positive regulation of MAPK cascade
positive regulation of mitotic nuclear division
positive regulation of transcription, DNA-templated
regulation of JAK-STAT cascade
system development
branching morphogenesis of an epithelial tube
epithelial cell proliferation
positive regulation of epithelial cell proliferation
negative regulation of secretion
regulation of transport
positive regulation of protein kinase B signaling
mammary gland alveolus development
ERK1 and ERK2 cascade
regulation of primary metabolic process
positive regulation of canonical Wnt signaling pathway
regulation of calcium ion import
negative regulation of cholesterol efflux
positive regulation of hyaluronan biosynthetic process
positive regulation of protein localization to early endosome
positive regulation of epithelial tube formation
regulation of protein localization to cell surface
positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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