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Invitrogen

EGF Polyclonal Antibody, Biotin, PeproTech®

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EGF Polyclonal Antibody, Biotin, PeproTech®

Product Details

500-P45BT-25UG

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.2 µg/mL
-

ELISA (ELISA)

0.25-1.0 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human EGF
View immunogen

Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Amount

25 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2930555

Product Specific Information

AA Sequence of recombinant protein: NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human EGF. Anti-Human EGF-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect hEGF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human EGF (500-P45) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hEGF.

Western Blot: To detect hEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hEGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P45BT-1MG will be provided as 2 x 500 µg

Target Information

EGF (Epidermal growth factor) exerts its actions by binding to the EGF Receptor, a 170 kDa protein kinase. Activation of EGFR initiates diverse cellular pathways in response to toxic environmental stimuli, or to EGF binding to the receptor, the EGFR forms homo- or heterodimers with other family members. Each dimeric receptor complex initiates a distinct signaling pathway by recruiting different Src homology 2 (SH2) containing effector proteins. EGF is far and wide expressed in kidney, cerebrum, prostrate and salivary glands. EGF acts as a potent mitogenic factor and the phosphorylated receptor recruits adapter proteins like GRB2 that activates complex downstream signaling cascades. EGF activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STAT modules. Research studies suggest the protein may also play important role in activating the NF-kappa-B signaling cascade. Defects in the EGGF gene are the cause of hypomagnesemia type 4 and dysregulation has been associated with the growth and progression of certain cancers.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: beta-urogastrone; EGF; Epidermal growth factor; H-EGF; Pro-epidermal growth factor; unnamed protein product

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Gene Aliases: EGF; HOMG4; URG

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UniProt ID: (Human) P01133

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Entrez Gene ID: (Human) 1950

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Function(s)
guanyl-nucleotide exchange factor activity epidermal growth factor receptor binding calcium ion binding protein binding growth factor activity transmembrane receptor protein tyrosine kinase activator activity receptor agonist activity
Process(es)
angiogenesis positive regulation of endothelial cell proliferation positive regulation of receptor internalization epidermal growth factor receptor signaling pathway cell proliferation positive regulation of cell proliferation positive regulation of endothelial cell migration positive regulation of macromolecule metabolic process positive regulation of gene expression positive regulation of peptidyl-threonine phosphorylation cerebellar granule cell precursor proliferation positive regulation of cerebellar granule cell precursor proliferation positive regulation of cell migration ERBB2-EGFR signaling pathway positive regulation of phosphorylation positive regulation of DNA binding positive regulation of MAPK cascade positive regulation of mitotic nuclear division positive regulation of transcription, DNA-templated regulation of JAK-STAT cascade system development branching morphogenesis of an epithelial tube epithelial cell proliferation positive regulation of epithelial cell proliferation negative regulation of secretion regulation of transport positive regulation of protein kinase B signaling mammary gland alveolus development ERK1 and ERK2 cascade regulation of primary metabolic process positive regulation of canonical Wnt signaling pathway regulation of calcium ion import negative regulation of cholesterol efflux positive regulation of hyaluronan biosynthetic process positive regulation of protein localization to early endosome positive regulation of epithelial tube formation regulation of protein localization to cell surface positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process
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