Western Blot: To detect hPDGF-AA by Western Blot analysis PDGF-AA Polyclonal Antibody, Biotin (Product # 500-P46BT-500UG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hPDGF-AA is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Product Image Gallery

Immunogen image

Immunogen Image PDGF-AA Polyclonal Antibody, Biotin, PeproTech®

Product Details

500-P46BT-50UG

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

ELISA (ELISA)

0.25-1.0 µg/mL

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived, 28.5 kDa Recombinant Human PDGF-AA

View immunogen

Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

50 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

AB_2930562

Product Specific Information

AA Sequence of recombinant protein: SIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATTSLN PDYREEDTGR PRESGKKRKR KRLKPT.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human PDGF-AA. Anti-Human PDGF-AA-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect hPDGF-AA by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human PDGF-AA (500-P46) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hPDGF-AA.

Western Blot: To detect hPDGF-AA by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hPDGF-AA is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P46BT-1MG will be provided as 2 x 500 µg

Target Information

Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the alpha -granules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGF-AA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed alpha and beta. Differences exist in isoform binding to each receptor. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. Other reported activities for PDGF include stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, inhibition of natural killer (NK) cell activity, stimulation of collagen synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-gamma production, temporary effects that may allow clonal expansion of antigen-activated B and T helper lymphocytes prior to differentiation. PDGF also appears to be ubiquitous in neurons throughout the CNS, where it is suggested to play an important role in neuron survival and regeneration, and in mediation of glial cell proliferation and differentiation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: H-PDGF-AA; PDGF A-chain; PDGF subunit A; PDGF-1; Platelet-derived growth factor A chain; platelet-derived growth factor A-chain; platelet-derived growth factor alpha chain; Platelet-derived growth factor alpha polypeptide; Platelet-derived growth factor subunit A; unnamed protein product

View more View less

Gene Aliases: PDGF-A; PDGF1; PDGFA

View more View less

UniProt ID: (Human) P04085

View more View less

Entrez Gene ID: (Human) 5154

View more View less

Function(s)

platelet-derived growth factor receptor binding protein binding collagen binding growth factor activity identical protein binding protein homodimerization activity protein heterodimerization activity receptor agonist activity platelet-derived growth factor binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

PDGF-AA Polyclonal Antibody, Biotin, PeproTech®

PDGF-AA Antibody (500-P46BT-50UG) in WB

Product Details

500-P46BT-50UG

Western Blot (WB)

ELISA (ELISA)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Amount

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!