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Invitrogen
IL-1 alpha Polyclonal Antibody, Biotin, PeproTech®
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FIGURE: 1 / 3
IL-1 alpha Antibody (500-P51ABT-25UG) in WB
Western Blot: To detect mIL-1alpha by Western Blot analysis IL-1 alpha Polyclonal Antibody, Biotin (Product # 500-P51ABT-500UG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mIL-1alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P51ABT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Murine IL-1alpha
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Amount
25 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: SAPYTYQSDL RYKLMKLVRQ KFVMNDSLNQ TIYQDVDKHY LSTTWLNDLQ QEVKFDMYAY SSGGDDSKYP VTLKISDSQL FVSAQGEDQP VLLKELPETP KLITGSETDL IFFWKSINSK NYFTSAAYPE LFIATKEQSR VHLARGLPSM TDFQIS.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-1alpha. Anti-Murine IL-1alpha-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect mIL-1alpha by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine IL-1alpha (500-P51A) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant mIL-1alpha.
Western Blot: To detect mIL-1alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mIL-1alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P51ABT-1MG will be provided as 2 x 500 µg
Target Information
IL-1 alpha (Interleukin-1 alpha) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 alpha is coded by the IL1A gene and signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 beta. IL1A is located on the q arm on chromosome 2 at position 13. IL-1 alpha plays an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses. IL-1 alpha activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. Further, IL-1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury and induces apoptosis. The IL1A gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. IL1 alpha regulates the activities of NF-kappaB and mitogen-activated protein kinases. IL-1 alpha can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-1 alpha are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CTLA8; IL 1 a; IL 1α; IL-1 alpha; IL17; IL1α; ILN; Interleukin; interleukin 17; Interleukin-1 alpha; Interleukin-17A; Interleukin1 alpha; RP23-160G19.8
Gene Aliases: Il-1a; Il1a
UniProt ID: (Mouse) P01582
Entrez Gene ID: (Mouse) 16175
fever generation
connective tissue replacement involved in inflammatory response wound healing
inflammatory response
immune response
negative regulation of cell proliferation
positive regulation of vascular endothelial growth factor production
positive regulation of steroid biosynthetic process
cytokine-mediated signaling pathway
keratinization
positive regulation of prostaglandin secretion
positive regulation of interleukin-6 production
positive regulation of stress-activated MAPK cascade
cellular response to heat
ectopic germ cell programmed cell death
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of interleukin-2 biosynthetic process
positive regulation of angiogenesis
positive regulation of mitotic nuclear division
positive regulation of transcription from RNA polymerase II promoter
positive regulation of JNK cascade
response to copper ion
positive regulation of cytokine secretion
positive regulation of cell division
regulation of sensory perception of pain
positive regulation of ERK1 and ERK2 cascade
extrinsic apoptotic signaling pathway in absence of ligand
positive regulation of neutrophil migration
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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