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IL-4 Polyclonal Antibody, PeproTech®
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IL-4 Antibody (500-P54-100UG) in WB
Western Blot: To detect Murine IL-4 by Western Blot analysis IL-4 Polyclonal Antibody (Product # 500-P54-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine IL-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
500-P54-100UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Human,
Mouse
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Murine IL-4
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: MHIHGCDKNH LREIIGILNE VTGEGTPCTE MDVPNVLTAT KNTTESELVC RASKVLRIFY LKHGKTPCLK KNSSVLMELQ RLFRAFRCLD SSISCTMNES KSTSLKDFLE SLKSIMQMDY S.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-4. Anti-Murine IL-4-specific antibody was purified by affinity chromatography employing an immobilized Murine IL-4 matrix.
Sandwich ELISA: To detect Murine IL-4 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine IL-4 (500-P54Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine IL-4.
Western Blot: To detect Murine IL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine IL-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: B-cell growth factor 1; B-cell IgG differentiation factor; B-cell stimulatory factor 1; H-IL-4; IGG1 induction factor; IL4; ILN; Interleukin; interleukin mRNA; Interleukin4; lymphocyte stimulatory factor 1; M-IL-4; MGC79402; unnamed protein product
Gene Aliases: BSF-1; Il-4
UniProt ID: (Mouse) P07750
Entrez Gene ID: (Mouse) 16189
negative regulation of transcription from RNA polymerase II promoter
microglial cell activation
positive regulation of protein phosphorylation
innate immune response in mucosa
positive regulation of defense response to virus by host
T-helper 1 cell lineage commitment
positive regulation of immunoglobulin production
negative regulation of acute inflammatory response
negative regulation of chronic inflammatory response
positive regulation of leukocyte chemotaxis
negative regulation of leukocyte activation
immune response
JAK-STAT cascade
cholesterol metabolic process
positive regulation of cell proliferation
response to nematode
regulation of proton transport
response to selenium ion
positive regulation of gene expression
negative regulation of epithelial cell migration
positive regulation of macroautophagy
positive regulation of cell migration
positive regulation of B cell proliferation
B cell costimulation
negative regulation of tumor necrosis factor production
positive regulation of chemokine production
positive regulation of interleukin-10 production
positive regulation of interleukin-13 production
interleukin-4-mediated signaling pathway
positive regulation of T cell proliferation
positive regulation of activated T cell proliferation
T cell activation
macrophage activation
positive regulation of tyrosine phosphorylation of STAT protein
defense response to protozoan
myeloid dendritic cell differentiation
negative regulation of macrophage activation
positive regulation of mast cell degranulation
negative regulation of nitric oxide biosynthetic process
T-helper 2 cell differentiation
leukocyte activation
positive regulation of MHC class II biosynthetic process
positive regulation of T cell differentiation
negative regulation of osteoclast differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of receptor-mediated endocytosis
positive regulation of isotype switching to IgE isotypes
positive regulation of isotype switching to IgG isotypes
negative regulation of inflammatory response
positive regulation of peptidyl-tyrosine phosphorylation
regulation of immune response
negative regulation of T cell activation
positive regulation of B cell activation
negative regulation of white fat cell proliferation
positive regulation of mononuclear cell migration
dendritic cell differentiation
extrinsic apoptotic signaling pathway in absence of ligand
positive regulation of cold-induced thermogenesis
neuroinflammatory response
positive regulation of beta-amyloid clearance
positive regulation of myoblast fusion
positive regulation of cellular respiration
positive regulation of reactive oxygen species biosynthetic process
negative regulation of complement-dependent cytotoxicity
negative regulation of cellular response to transforming growth factor beta stimulus
negative regulation of T-helper 17 cell differentiation
negative regulation of endothelial cell apoptotic process
positive regulation of eosinophil chemotaxis
positive regulation of B cell chemotaxis
positive regulation of T-helper 2 cell cytokine production
positive regulation of ATP biosynthetic process
negative regulation of extrinsic apoptotic signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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