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Invitrogen

RANKL (soluble) Polyclonal Antibody, PeproTech®

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RANKL (soluble) Polyclonal Antibody, PeproTech®

Product Details

500-P63-1MG

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.2 µg/mL
-

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

ELISA (ELISA)

0.5-2.0 µg/mL
-
Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Murine sRANK Ligand
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Amount

1 mg

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2930666

Product Specific Information

AA Sequence of recombinant protein: PAMMEGSWLD VAQRGKPEAQ PFAHLTINAA SIPSGSHKVT LSSWYHDRGW AKISNMTLSN GKLRVNQDGF YYLYANICFR HHETSGSVPT DYLQLMVYVV KTSIKIPSSH NLMKGGSTKN WSGNSEFHFY SINVGGFFKL RAGEEISIQV SNPSLLDPDQ DATYFGAFKV QDID.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine sRANK Ligand. Anti-Murine sRANK Ligand-specific antibody was purified by affinity chromatography employing an immobilized Murine sRANK Ligand matrix.

Sandwich ELISA: To detect Murine sRANK Ligand by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine sRANKL (500-P63Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine sRANK Ligand.

Western Blot: To detect Murine sRANK Ligand by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine sRANK Ligand is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CD254; H-RANK-L; M-Rank-L; ODF; OPG ligand; OPGL; Osteoclast differentiation factor; Osteoprotegerin ligand; rank-l; RANKL; receptor activator of NF-kappaB ligand; receptor activator of nuclear factor kappa B ligand; Receptor activator of nuclear factor kappa-B ligand; RP11-86N24.2; srankl; TNF-related activation-induced cytokine; TRANCE; Tumor necrosis factor ligand superfamily member 11; tumor necrosis factor-related activation-induced cytokine

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Gene Aliases: Ly109l; ODF; OPG; OPGL; RANKL; Tnfsf11; Trance

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UniProt ID: (Mouse) O35235

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Entrez Gene ID: (Mouse) 21943

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Function(s)
cytokine activity tumor necrosis factor receptor binding protein binding tumor necrosis factor receptor superfamily binding
Process(es)
ossification osteoclast proliferation monocyte chemotaxis immune response activation of JUN kinase activity multicellular organism development organ morphogenesis cytokine-mediated signaling pathway cell differentiation osteoclast differentiation tumor necrosis factor-mediated signaling pathway mammary gland epithelial cell proliferation positive regulation of homotypic cell-cell adhesion paracrine signaling positive regulation of phosphorylation positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of MAP kinase activity bone resorption regulation of osteoclast differentiation positive regulation of osteoclast differentiation positive regulation of bone resorption positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade lymph node development positive regulation of T cell activation positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of NF-kappaB transcription factor activity protein homooligomerization positive regulation of corticotropin-releasing hormone secretion positive regulation of protein kinase B signaling calcium ion homeostasis mammary gland alveolus development ERK1 and ERK2 cascade positive regulation of fever generation by positive regulation of prostaglandin secretion TNFSF11-mediated signaling pathway positive regulation of ERK1 and ERK2 cascade via TNFSF11-mediated signaling positive regulation of intracellular signal transduction positive regulation of osteoclast development
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