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          • Primary Antibodies ›
          • TNF alpha Antibodies

          Invitrogen

          TNF alpha Polyclonal Antibody, Biotin, PeproTech®

          View all (222) TNF alpha antibodies

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          Cite TNF alpha Polyclonal Antibody, Biotin, PeproTech®

          Additional Information:
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          • Antibody Testing Data (3)
          TNF alpha Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          TNF alpha Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          TNF alpha Antibody (500-P72BT-500UG) in WB

          Western Blot: To detect Rat TNF-alpha by Western Blot analysis TNF alpha Polyclonal Antibody, Biotin (Product # 500-P72BT-500UG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Rat TNF-alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          View Product

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          TNF alpha Antibody in Western Blot (WB)
          TNF alpha Antibody in Western Blot (WB)
          TNF alpha Antibody in ELISA (ELISA)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          TNF alpha Polyclonal Antibody, Biotin, PeproTech®

          Product Details

          500-P72BT-500UG

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.1-0.2 µg/mL
          -

          ELISA (ELISA)

          0.25-1.0 µg/mL
          -
          Product Specifications

          Species Reactivity

          Rat

          Host/Isotype

          Rabbit

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          E.coli-derived, 17.3 kDa Recombinant Rat TNF-alpha
          3D Epitope / Immunogen

          Conjugate

          Biotin Biotin Biotin

          Form

          Lyophilized

          Concentration

          0.1-1.0 mg/mL

          Amount

          500 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS

          Contains

          no preservative

          Storage conditions

          -20°C

          Shipping conditions

          Ambient

          RRID

          AB_3251327

          Product Specific Information

          AA Sequence of recombinant protein: MLRSSSQNSS DKPVAHVVAN HQAEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLIY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VSLLSAIKSP CPKDTPEGAE LKPWYEPMYL GGVFQLEKGD LLSAEVNLPK YLDITESGQV YFGVIAL.

          Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Rat TNF-alpha. Anti-Rat TNF-alpha-specific antibody was purified by affinity chromatography and then biotinylated.

          Sandwich ELISA: To detect Rat TNF-alpha by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Rat TNF-alpha (500-P72) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat TNF-alpha.

          Western Blot: To detect Rat TNF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Rat TNF-alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

          500-P72BT-1MG will be provided as 2 x 500 µg

          Target Information

          TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: cachectin; DADB-70P7.1; TNF alpha; TNF superfamily; TNF superfamily member 2; TNF α; TNFα; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor ligand superfamily member 2

          View more View less

          Gene Aliases: RATTNF; TNF-alpha; Tnfa

          View more View less

          UniProt ID: (Rat) P16599

          View more View less

          Entrez Gene ID: (Rat) 24835

          View more View less

          Function(s)
          transcription regulatory region sequence-specific DNA binding protease binding cytokine activity tumor necrosis factor receptor binding identical protein binding receptor agonist activity histone H3K9ac reader activity
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter response to hypoxia microglial cell activation positive regulation of cytokine production regulation of protein phosphorylation negative regulation of endothelial cell proliferation response to amphetamine negative regulation of L-glutamate transport macrophage activation involved in immune response chronic inflammatory response to antigenic stimulus regulation of immunoglobulin production positive regulation of immunoglobulin production positive regulation of immune effector process negative regulation of cytokine production involved in immune response positive regulation of chronic inflammatory response to antigenic stimulus positive regulation of humoral immune response mediated by circulating immunoglobulin skeletal muscle contraction negative regulation of systemic arterial blood pressure glucose metabolic process chromatin organization defense response inflammatory response immune response humoral immune response JNK cascade JAK-STAT cascade regulation of mitotic cell cycle circadian rhythm positive regulation of cell proliferation negative regulation of cell proliferation extrinsic apoptotic signaling pathway via death domain receptors intrinsic apoptotic signaling pathway in response to DNA damage response to xenobiotic stimulus response to mechanical stimulus response to virus response to bacterium response to fructose organ morphogenesis negative regulation of heart rate vascular endothelial growth factor production positive regulation of gene expression negative regulation of gene expression positive regulation of macrophage derived foam cell differentiation response to activity calcium-mediated signaling extracellular matrix organization osteoclast differentiation positive regulation of protein complex assembly positive regulation of defense response positive regulation of fever generation negative regulation of myelination lipopolysaccharide-mediated signaling pathway response to nutrient levels response to lipopolysaccharide negative regulation of interleukin-6 production positive regulation of chemokine production positive regulation of fractalkine production positive regulation of interferon-gamma production positive regulation of interleukin-1 beta production positive regulation of interleukin-18 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production tumor necrosis factor-mediated signaling pathway positive regulation of heterotypic cell-cell adhesion toll-like receptor 3 signaling pathway response to isolation stress response to macrophage colony-stimulating factor regulation of cell proliferation vasodilation defense response to bacterium positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of programmed cell death regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of I-kappaB kinase/NF-kappaB signaling response to amino acid positive regulation of protein complex disassembly positive regulation of MAPK cascade positive regulation of neuron apoptotic process negative regulation of blood vessel endothelial cell migration negative regulation of viral genome replication cellular extravasation positive regulation of nitric oxide biosynthetic process response to ethanol regulation of fat cell differentiation negative regulation of fat cell differentiation negative regulation of myoblast differentiation negative regulation of osteoblast differentiation regulation of osteoclast differentiation positive regulation of osteoclast differentiation positive regulation of protein catabolic process positive regulation of action potential positive regulation of cell adhesion positive regulation of mitotic nuclear division negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated negative regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter positive regulation of translational initiation by iron negative regulation of glucose import positive regulation of JNK cascade response to antibiotic embryonic digestive tract development positive regulation of smooth muscle cell proliferation regulation of epithelial cell proliferation regulation of protein secretion regulation of inflammatory response regulation of developmental process regulation of insulin secretion modulation of synaptic transmission positive regulation of synaptic transmission regulation of synapse organization defense response to Gram-positive bacterium leukocyte migration leukocyte tethering or rolling detection of mechanical stimulus involved in sensory perception of pain negative regulation of lipid catabolic process positive regulation of nitric-oxide synthase activity positive regulation of membrane protein ectodomain proteolysis regulation of secretion positive regulation of protein transport response to glucocorticoid positive regulation of hair follicle development positive regulation of protein kinase B signaling positive regulation of glial cell proliferation positive regulation of vitamin D biosynthetic process epithelial cell proliferation involved in salivary gland morphogenesis regulation of branching involved in salivary gland morphogenesis negative regulation of vascular wound healing negative regulation of branching involved in lung morphogenesis positive regulation of calcineurin-NFAT signaling cascade cellular response to molecule of bacterial origin cellular response to lipopolysaccharide cellular response to amino acid stimulus cellular response to retinoic acid cellular response to nicotine cellular response to interferon-gamma cellular response to ionizing radiation positive regulation of podosome assembly endothelial cell apoptotic process protein localization to plasma membrane negative regulation of oxidative phosphorylation inflammatory response to wounding apoptotic signaling pathway extrinsic apoptotic signaling pathway cellular response to toxic substance liver regeneration necroptotic signaling pathway negative regulation of bile acid secretion antiviral innate immune response response to Gram-negative bacterium positive regulation of interleukin-33 production positive regulation of cytokine production involved in inflammatory response negative regulation of beta-amyloid clearance positive regulation of NIK/NF-kappaB signaling positive regulation of synoviocyte proliferation positive regulation of beta-amyloid formation response to L-glutamate positive regulation of neutrophil activation positive regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of protein localization to plasma membrane regulation of establishment of endothelial barrier negative regulation of bicellular tight junction assembly response to resveratrol cellular response to beta-amyloid positive regulation of vascular smooth muscle cell proliferation response to hydrogen sulfide positive regulation of leukocyte adhesion to vascular endothelial cell positive regulation of leukocyte adhesion to arterial endothelial cell response to quercetin response to 3,3',5-triiodo-L-thyronine response to gold nanoparticle positive regulation of protein localization to cell surface positive regulation of blood microparticle formation positive regulation of chemokine (C-X-C motif) ligand 2 production positive regulation of hepatocyte proliferation regulation of endothelial cell apoptotic process positive regulation of endothelial cell apoptotic process regulation of reactive oxygen species metabolic process positive regulation of DNA biosynthetic process positive regulation of estradiol secretion negative regulation of apoptotic signaling pathway positive regulation of extrinsic apoptotic signaling pathway negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
          It has to be done as per old AB suggested Products section.

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