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Invitrogen
CNTF Polyclonal Antibody, Biotin, PeproTech®
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FIGURE: 1 / 3
CNTF Antibody (500-P79BT-50UG) in WB
Western Blot: To detect Rat CNTF by Western Blot analysis CNTF Polyclonal Antibody, Biotin (Product # 500-P79BT-500UG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat CNTF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P79BT-50UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Rat CNTF
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: AFAEQTPLTL HRRDLCSRSI WLARKIRSDL TALMESYVKH QGLNKNINLD SVDGVPVAST DRWSEMTEAE RLQENLQAYR TFQGMLTKLL EDQRVHFTPT EGDFHQAIHT LMLQVSAFAY QLEELMVLLE QKIPENEADG MPATVGDGGL FEKKLWGLKV LQELSQWTVR SIHDLRVISS HQMGISALES HYGAKDKQM.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Rat CNTF. Anti-Rat CNTF-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Rat CNTF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Rat CNTF (500-P79) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat CNTF.
Western Blot: To detect Rat CNTF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat CNTF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P79BT-1MG will be provided as 2 x 500 µg
Target Information
The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. A read-through transcript variant composed of ZFP91 and CNTF sequence has been identified, but it is thought to be non-coding. Read-through transcription of ZFP91 and CNTF has also been observed in mouse.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Ciliary neurotrophic factor; ciliary neurotropic factor; CNTF; H-CNTF
Gene Aliases: Cntf
UniProt ID: (Rat) P20294
Entrez Gene ID: (Rat) 25707
JAK-STAT cascade
nervous system development
positive regulation of cell proliferation
response to light intensity
positive regulation of phosphatidylinositol 3-kinase signaling
positive regulation of cell growth
growth
positive regulation of tyrosine phosphorylation of Stat3 protein
positive regulation of MAPK cascade
negative regulation of neuron apoptotic process
positive regulation of JAK-STAT cascade
negative regulation of photoreceptor cell differentiation
regulation of retinal cell programmed cell death
positive regulation of hormone secretion
astrocyte activation
muscle organ morphogenesis
neuron development
positive regulation of axon regeneration
protein heterooligomerization
response to electrical stimulus
regulation of resting membrane potential
membrane hyperpolarization
ciliary neurotrophic factor-mediated signaling pathway
negative regulation of neuron death
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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