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          • Primary Antibodies ›
          • IL-1 beta Antibodies

          Invitrogen

          IL-1 beta Polyclonal Antibody, Biotin, PeproTech®

          View all (118) IL-1 beta antibodies

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          Cite IL-1 beta Polyclonal Antibody, Biotin, PeproTech®

          Additional Information:

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          • Antibody Testing Data (3)
          IL-1 beta Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          IL-1 beta Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          IL-1 beta Antibody (500-P80BT-50UG) in WB

          Western Blot: To detect Rat IL-1beta by Western Blot analysis IL-1 beta Polyclonal Antibody, Biotin (Product # 500-P80BT-500UG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat IL-1beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          IL-1 beta Antibody in Western Blot (WB)
          IL-1 beta Antibody in Western Blot (WB)
          IL-1 beta Antibody in ELISA (ELISA)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          IL-1 beta Polyclonal Antibody, Biotin, PeproTech®

          Product Details

          500-P80BT-50UG

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.1-0.2 µg/mL
          -

          ELISA (ELISA)

          0.25-1.0 µg/mL
          -
          Product Specifications

          Species Reactivity

          Rat

          Host/Isotype

          Rabbit

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          E.coli-derived Recombinant Rat IL-1beta
          3D Epitope / Immunogen

          Conjugate

          Biotin Biotin Biotin

          Form

          Lyophilized

          Concentration

          0.1-1.0 mg/mL

          Amount

          50 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS

          Contains

          no preservative

          Storage conditions

          -20°C

          Shipping conditions

          Ambient

          RRID

          AB_2930778

          Product Specific Information

          AA Sequence of recombinant protein: MVPIRQLHCR LRDEQQKCLV LSDPCELKAL HLNGQNISQQ VVFSMSFVQG ETSNDKIPVA LGLKGLNLYL SCVMKDGTPT LQLESVDPKQ YPKKKMEKRF VFNKIEVKTK VEFESAQFPN WYISTSQAEH RPVFLGNSNG RDIVDFTMEP VSS.

          Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Rat IL-1beta. Anti-Rat IL-1beta-specific antibody was purified by affinity chromatography and then biotinylated.

          Sandwich ELISA: To detect Rat IL-1beta by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Rat IL-1beta (500-P80) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat IL-1beta.

          Western Blot: To detect Rat IL-1beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat IL-1beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

          500-P80BT-1MG will be provided as 2 x 500 µg

          Target Information

          Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: Hematopoietin 1 (H1); IFN beta inducing factor; il 1b; IL 1β; IL-1 beta; IL1 B; IL1β; ILN; Interleukin; Interleukin 1 beta precursor; Interleukin-1b; Interleukin1 beta; LAF; Osteoclast activating factor (OAF); Pro interleukin 1 beta

          View more View less

          Gene Aliases: IL-1F2

          View more View less

          Entrez Gene ID: (Rat) 24494

          View more View less

          Function(s)
          cytokine activity interleukin-1 receptor binding integrin binding protein domain specific binding receptor agonist activity
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter fever generation response to hypoxia response to amphetamine chronic inflammatory response to antigenic stimulus inflammatory response immune response positive regulation of cytosolic calcium ion concentration response to nutrient learning or memory memory positive regulation of cell proliferation negative regulation of cell proliferation response to xenobiotic stimulus response to carbohydrate response to ozone response to gamma radiation vascular endothelial growth factor production positive regulation of vascular endothelial growth factor production positive regulation of gene expression negative regulation of gene expression positive regulation of platelet-derived growth factor receptor signaling pathway positive regulation of epithelial to mesenchymal transition positive regulation of macrophage derived foam cell differentiation negative regulation of glucose transport negative regulation of glutamate secretion cytokine-mediated signaling pathway hyaluronan biosynthetic process positive regulation of cell migration neutrophil chemotaxis positive regulation of prostaglandin biosynthetic process positive regulation of fever generation lipopolysaccharide-mediated signaling pathway positive regulation of prostaglandin secretion response to estradiol response to lipopolysaccharide positive regulation of chemokine production positive regulation of granulocyte macrophage colony-stimulating factor production positive regulation of interferon-gamma production positive regulation of interleukin-2 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of stress-activated MAPK cascade regulation of organic acid transport positive regulation of immature T cell proliferation in thymus response to ATP response to vitamin D response to L-ascorbic acid positive regulation of heterotypic cell-cell adhesion social behavior ectopic germ cell programmed cell death response to stilbenoid response to isolation stress response to immobilization stress positive regulation of T cell proliferation positive regulation of apoptotic process regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of MAPK cascade positive regulation of MAPK cascade response to peptide hormone positive regulation of JUN kinase activity positive regulation of neuron apoptotic process positive regulation of nitric oxide biosynthetic process response to ethanol negative regulation of neuron differentiation positive regulation of glial cell differentiation positive regulation of angiogenesis negative regulation of lipid metabolic process positive regulation of mitotic nuclear division positive regulation of transcription, DNA-templated positive regulation of complement activation positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade negative regulation of insulin receptor signaling pathway astrocyte activation positive regulation of astrocyte differentiation regulation of defense response to virus by host positive regulation of inflammatory response regulation of neurogenesis negative regulation of neurogenesis regulation of insulin secretion negative regulation of synaptic transmission defense response to Gram-positive bacterium leukocyte migration negative regulation of lipid catabolic process positive regulation of lipid catabolic process regulation of nitric-oxide synthase activity positive regulation of membrane protein ectodomain proteolysis response to glucocorticoid positive regulation of protein kinase B signaling positive regulation of glial cell proliferation negative regulation of adiponectin secretion regulation of ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade monocyte aggregation interleukin-1-mediated signaling pathway response to interleukin-1 cellular response to lipopolysaccharide cellular response to antibiotic cellular response to mechanical stimulus cellular response to glucose stimulus cellular response to lipid cellular response to fatty acid cellular response to xenobiotic stimulus response to dexamethasone positive regulation of monocyte chemotactic protein-1 production positive regulation of neutrophil chemotaxis extrinsic apoptotic signaling pathway in absence of ligand cellular response to interleukin-17 positive regulation of p38MAPK cascade positive regulation of NIK/NF-kappaB signaling positive regulation of RNA biosynthetic process regulation of establishment of endothelial barrier negative regulation of secretion by cell negative regulation of gap junction assembly response to phorbol 13-acetate 12-myristate response to hydrogen sulfide positive regulation of occluding junction disassembly negative regulation of branching morphogenesis of a nerve negative regulation of neural precursor cell proliferation positive regulation of T-helper 1 cell cytokine production negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
          It has to be done as per old AB suggested Products section.

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