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Invitrogen
NTF4 Polyclonal Antibody, Biotin, PeproTech®
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Product Details
500-P83GBT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived, 28.1 kDa Recombinant Human NT-4
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Amount
25 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: GVSETAPASR RGELAVCDAV SGWVTDRRTA VDLRGREVEV LGEVPAAGGS PLRQYFFETR CKADNAEEGG PGAGGGGCRG VDRRHWVSEC KAKQSYVRAL TADAQGRVGW RWIRIDTACV CTLLSRTGRA.
Preparation: Produced from sera of goats immunized with highly pure Recombinant Human NT-4. Anti-Human NT-4-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hNT-4 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human NT-4 (500-P83G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hNT-4.
Western Blot: To detect hNT-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hNT-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P83GBT-1MG will be provided as 2 x 500 µg
Target Information
This gene is a member of a family of neurotrophic factors, neurotrophins, that control survival and differentiation of mammalian neurons. The expression of this gene is ubiquitous and less influenced by environmental signals. While knock-outs of other neurotrophins including nerve growth factor, brain-derived neurotrophic factor, and neurotrophin 3 prove lethal during early postnatal development, NTF5-deficient mice only show minor cellular deficits and develop normally to adulthood.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: neurotrophic factor 4; neurotrophic factor 5; Neurotrophin 4/5; neurotrophin 5 (neurotrophin 4/5); Neurotrophin-4; Neurotrophin-5; Neutrophic factor 4; Neutrophic factor 5; NT-4; NT-5; NT4/NT5; NTF5; unnamed protein product
Gene Aliases: GLC10; GLC1O; NT-4; NT-4/5; NT-5; NT4; NT5; NTF4; NTF5
UniProt ID: (Human) P34130
Entrez Gene ID: (Human) 4909
transmembrane receptor protein tyrosine kinase signaling pathway
long-term memory
sensory organ boundary specification
adult locomotory behavior
epidermis development
nerve development
ameloblast differentiation
nerve growth factor signaling pathway
mechanoreceptor differentiation
negative regulation of programmed cell death
negative regulation of neuron apoptotic process
neuron projection morphogenesis
modulation of synaptic transmission
innervation
taste bud development
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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