Search

  • Order Status
  • Quick Order
  • Promos

Invitrogen

CX3CL1 Polyclonal Antibody, Biotin, PeproTech®

View all (33) CX3CL1 antibodies

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Additional Information:
{{banner.description}}
{{$ctrl.isSkuNotesCollapsed ? 'View more' : 'View less'}}
CX3CL1 Polyclonal Antibody, Biotin, PeproTech®

Product Details

500-P98BT-50UG

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.2 µg/mL
-

ELISA (ELISA)

0.25-1.0 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human Fractalkine (CX3CL1)
View immunogen

Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Amount

50 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2930898

Product Specific Information

AA Sequence of recombinant protein: QHHGVTKCNI TCSKMTSKIP VALLIHYQQN QASCGKRAII LETRQHRLFC ADPKEQWVKD AMQHLDRQAA ALTRNG.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Fractalkine (CX3CL1). Anti-Human Fractalkine (CX3CL1)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect hFractalkine by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Fractalkine (500-P98) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hFractalkine.

Western Blot: To detect hFractalkine by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hFractalkine is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P98BT-1MG will be provided as 2 x 500 µg

Target Information

Chemokines are a family of proteins associated with the trafficking of leukocytes in immune surveillance and inflammatory cell recruitment. They are classified based on the positions of key cysteine residues. CX3CL1 is a CX3C chemokine known to induce adhesion and migration of leukocytes mediated by a membrane-bound and soluble form respectively. Recent experiments have shown that CX3CL1 can suppress the production of nitrous oxide, interleukin-6, and TNF-a in activated microglia and neuronal cells, suggesting that it may act as an intrinsic inhibitor against neurotoxicity by activated microglia. Its receptor, CX3CR1, also functions as a co-receptor for HIV-1 and HIV-2 envelope fusion and virus infection, which can be inhibited by CX3CL1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?
Let us know so we can reference it here.
Cite this product

Bioinformatics

Protein Aliases: C-X3-C motif chemokine 1; chemokine (C-X3-C motif) ligand 1; CX3C membrane-anchored chemokine; CX3CL1; Fractalkine; Neurotactin; small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin); small inducible cytokine subfamily D (Cys-X3-Cys), member-1; Small-inducible cytokine D1; unnamed protein product

View more View less

Gene Aliases: A-152E5.2; ABCD-3; C3Xkine; CX3CL1; CXC3; CXC3C; FKN; fractalkine; neurotactin; NTN; NTT; SCYD1

View more View less

UniProt ID: (Human) P78423

View more View less

Entrez Gene ID: (Human) 6376

View more View less

Function(s)
receptor binding cytokine activity integrin binding protein binding chemokine activity CX3C chemokine receptor binding chemoattractant activity CXCR1 chemokine receptor binding CCR chemokine receptor binding
Process(es)
microglial cell activation positive regulation of cell-matrix adhesion positive regulation of neuroblast proliferation leukocyte migration involved in inflammatory response response to ischemia chemotaxis defense response inflammatory response immune response cell adhesion G-protein coupled receptor signaling pathway cell-cell signaling positive regulation of cell proliferation negative regulation of cell-substrate adhesion positive regulation of neuron projection development neuron remodeling cytokine-mediated signaling pathway positive regulation of cell migration negative regulation of cell migration neutrophil chemotaxis leukocyte chemotaxis regulation of lipopolysaccharide-mediated signaling pathway positive regulation of actin filament bundle assembly negative regulation of interleukin-1 alpha production negative regulation of interleukin-1 beta production negative regulation of interleukin-6 production negative regulation of tumor necrosis factor production positive regulation of transforming growth factor beta1 production integrin activation autocrine signaling wound healing negative regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of MAPK cascade positive regulation of angiogenesis positive regulation of transcription from RNA polymerase II promoter regulation of synaptic plasticity eosinophil chemotaxis macrophage chemotaxis lymphocyte chemotaxis positive regulation of smooth muscle cell proliferation positive regulation of inflammatory response regulation of neurogenesis leukocyte adhesive activation positive chemotaxis positive regulation of calcium-independent cell-cell adhesion positive regulation of release of sequestered calcium ion into cytosol positive regulation of protein kinase B signaling angiogenesis involved in wound healing cell chemotaxis microglial cell proliferation antimicrobial humoral immune response mediated by antimicrobial peptide neuron cellular homeostasis chemokine-mediated signaling pathway positive regulation of ERK1 and ERK2 cascade extrinsic apoptotic signaling pathway in absence of ligand cell-cell adhesion synapse pruning negative regulation of hippocampal neuron apoptotic process negative regulation of glutamate receptor signaling pathway negative regulation of microglial cell activation positive regulation of microglial cell migration negative regulation of neuron migration negative regulation of apoptotic signaling pathway negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support