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Sendai virus HN Monoclonal Antibody (1A6), Alexa Fluor™ 647, eBioscience™
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FIGURE: 1 / 4
Sendai virus HN Antibody (51-6494-82) in ICC/IF
Immunofluorescence analysis of Sendai virus HN Monoclonal Antibody (Product # 51-6494-82) was performed using 70% confluent log phase HeLa cells transduced with Sendai virus. The cells were fixed with 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 30 minutes at 37C, and blocked with 10% Normal Goat Serum for 1 hour at room temperature. The cells were labeled... View More
Product Details
51-6494-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Virus
Host/Isotype
Mouse
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
1A6
Immunogen
Sendai Virus
Conjugate
Excitation/Emission Max
650/671 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This 1A6 monoclonal antibody recognizes hemagglutinin-neuraminidase of Sendai virus. This clone has been successfully used in flow cytometry, immunocytochemistry and has been reported for neutralization. The CTS CytoTune-iPS 2.1 Sendai Reprogramming Kit and the CytoTune-iPS 2.0 Sendai Reprograming Kit utilize Sendai virus particles to deliver Yamanaka factors into target cells in order to reprogram them into induced Pluripotent Stem Cells (iPSC). Clone 1A6 can be used to detect the presence of the residual Sendai virus after transduction and subsequent tissue culture.
Applications Reported: This 1A6 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 1A6 antibody has been tested by flow cytometric analysis of Sendai virus-infected cells. This may be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
Target Information
Sendai virus is a single stranded RNA respirovirus from the family Paramyxoviridae, which replicates in the respiratory tract and infects certain mammals, including mice, hamsters, rats and guinea pigs. The virus is highly contagious and in the wild is transmitted through contact with aerosolized respiratory secretions. The Hemagglutinin-Neuraminidase (HN) protein of the Sendai virus facilitates cell entry and infection by binding and cleaving terminal sialic acids present on many cell surface glycoproteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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