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Invitrogen
MCL-1 Monoclonal Antibody (LVUBKM), Alexa Fluor™ 647, eBioscience™
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FIGURE: 1 / 1
MCL-1 Antibody (51-9047-42) in Flow
Normal human peripheral blood cells were untreated (blue histogram) or stimulated for 72 hours with CD3 and CD28 Monoclonal Antibodies, Functional Grade (Product # 16-0037-85 and Product # 16-0289-85) and MG-132 (purple histogram). Cells were then stained intracellularly, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, with MCL-1 Monoclonal Antibody, Alexa Fluor 647. Total viable lymphocytes were used for analysis, as determined by Fixable Viability Dye eFluor 780 (Product # 65-0865-18).
Product Details
51-9047-42
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
LVUBKM
Conjugate
Excitation/Emission Max
650/671 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This LVUBKM monoclonal antibody recognizes human and mouse myeloid cell leukemia sequence 1 (Mcl-1). Mcl-1 is an anti-apoptotic member of the Bcl-2 family of proteins important for regulation of cell survival/apoptosis. Mcl-1 is primarily localized to the outer membrane of mitochondria where it prevents cytochrome c release via dimerization with other Bcl-2 family members such as Bim. Although it is expressed in both immune and non-immune cells, highest levels of Mcl-1 expression are seen in hematopoietic lineage cells. PI3K activation of AKT results in destabilization and degradation of GSK3 beta, which prevents phosphorylation of Mcl-1 on S159 and its subsequent ubiquitnation and degradation. Mice conditionally lacking Mcl-1 in lymphocytes showed that Mcl-1 is essential during early lymphoid development and for the maintenance of mature lymphocytes.
Applications Reported: This LVUBKM antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This LVUBKM antibody has been pre-diluted and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol A: Two step protocol for intracellular (cytoplasmic) proteins" located at www.thermofisher.com/flowprotocols . This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Target Information
MCL1 (Myeloid cell leukemia-1) belongs to the Bcl-2 family and is involved in programing, differentiation and concomitant maintenance of cell viability, but not of proliferation. Isoform 1 of MCL1 inhibits apoptosis while isoform 2 promotes it. The carboxy terminal of MCL1 and bcl-2 share significant sequence homology. Expression of MCL1 is increased upon exposure of ML-1 cells to various types of DNA damaging agents (e.g. ionizing radiation, ultraviolet radiation, and alkylating drugs) along with increases in GADD45 and Bax and a decrease in bcl-2. Enhanced expression of MCL1, prominently associated with mitochondria, complements the continued expression of bcl-2 in ML-1 cells undergoing differentiation. Like bcl-2, MCL1 has the capacity to promote cell viability under conditions that otherwise cause apoptosis. While the mechanism by which MCL1 inhibits apoptosis is not known, it is thought that it may heterodimerize and neutralize pro-apoptotic members of the Bcl-2 family such as Bim or Bak. MCL1 was originally identified in differentiating myeloid cells, but has since been shown to be expressed in multiple cell types. MCL1 is essential for embryogenesis and for the development and maintenance of B and T lymphocytes in animals. MCL1 exists as at least two distinct isoforms designated MCL1L and MCL1S. In marked contrast to the larger isoform of MCL1, overexpression of MCL1S promotes cell death.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: anti-apoptotic; Bcl-2-like protein 3; Bcl-2-related protein EAT/mcl1; BCL2 family apoptosis regulator; Bcl2-L-3; Induced myeloid leukemia cell differentiation protein Mcl-1; Induced myeloid leukemia cell differentiation protein Mcl-1 homolog; MCL-1ES; MCL-1S; Mcl-1S; Bcl-2 family protein; proapoptotic protein; MCL1, BCL2 family apoptosis regulator; mcl1/EAT; MGC104264; MGC1839; myeloid cell leukemia 1; myeloid cell leukemia ES; myeloid cell leukemia sequence 1 (BCL2-related); unnamed protein product
Gene Aliases: AW556805; bcl2-L-3; BCL2L3; EAT; Mcl-1; MCL1; MCL1-ES; mcl1/EAT; MCL1L; MCL1S; TM
UniProt ID: (Human) Q07820, (Mouse) P97287
Entrez Gene ID: (Human) 4170, (Mouse) 17210
apoptotic process
multicellular organism development
intrinsic apoptotic signaling pathway in response to DNA damage
apoptotic mitochondrial changes
cell differentiation
response to cytokine
regulation of apoptotic process
negative regulation of apoptotic process
extrinsic apoptotic signaling pathway in absence of ligand
positive regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway
negative regulation of anoikis
regulation of response to DNA damage stimulus
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
negative regulation of intrinsic apoptotic signaling pathway
cell fate determination
release of cytochrome c from mitochondria
cellular response to DNA damage stimulus
mitochondrial fusion
negative regulation of autophagy
cellular homeostasis
positive regulation of apoptotic process
positive regulation of neuron apoptotic process
transmembrane transport
protein transmembrane transport
regulation of intracellular signal transduction
regulation of apoptotic signaling pathway
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