Search
Invitrogen
Axl Monoclonal Antibody (MAXL8DS), Alexa Fluor™ 488, eBioscience™
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 3
Axl Antibody (53-1084-80) in Flow
Mouse bone marrow cells were cultured in the presence of GM-CSF for 7 days, Fc blocked with CD16/CD32 Antibody (Product # 14-0161-82) and normal mouse serum, and subsequently stained with CD11b Monoclonal Antibody, PE (Product # 12-0112-82) and 0.06 µg of Rat IgG2a kappa Isotype Control, Alexa Fluor 488 (Product # 53-4321-82) (left) or 0.06 µg of Axl Monoclonal Antibody, Alexa Fluor 488 (right). Viable cells, as determined by Fixable Viability Dye eFluor 450 (Product # 65-0863-18), in the large scatter gate were used for analysis.
Product Details
53-1084-80
Product Specifications
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
MAXL8DS
Immunogen
C57BL/6 mouse Axl-human IgG Fc fusion protein
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
0.5 mg/mL
Amount
25 µg
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
Product Specific Information
Description: This MAXL8DS monoclonal antibody recognizes mouse Axl, a member of the TAM family of tyrosine kinase receptors that also includes MerTK and Tyro3. Within hematopoietic compartment the highest expression of Axl can be observed on dendritic cells and macrophages. Stimulation with TLR ligands has been shown to additionally upregulate this expression. This MAXL8DS antibody will work in flow cytometry on both native and fixed/permeabilized cells.
Applications Reported: This MAXL8DS antibody has been reported for use in flow cytometric analysis.
Applications Tested: This MAXL8DS antibody has been tested by flow cytometric analysis of mouse bone marrow cells cultured with GM-CSF. This may be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser
Target Information
AXL is a chronic myelogenous leukemia-associated oncogene that is also associated with colon cancer and melanoma. The AXL protein is a receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6, and is involved in the stimulation of cell proliferation. TAM receptors are also involved in phagocytosis of apoptotic cells. Mice lacking all three TAM receptors have several degenerative phenotypes linked to inefficient removal of apoptotic cells and membranes (e.g., in the retina and the male reproductive tract) and develop a severe autoimmune phenotype akin to systemic lupus erythematosus, including the production of broad spectrum auto-antibodies. In addition, Axl may function as a putative entry receptor for filoviruses. Axl is also used to identify a specific subpopulation of human blood dendritic cells, also referred to as Siglec 6+/Axl+ dendritic cells. Cellular expression of Axl can be upregulated by TLR ligands, such as LPS or poly I:C. Soluble Axl is generated by proteolytic cleavage of the membrane form. Increased plasma levels may be indicative of inflammation and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support